Description

Azido-Peg1-Pfp Ester is a heterobifunctional crosslinking reagent featuring a reactive pentafluorophenyl (PFP) ester and a terminal azide group. This compound is specifically engineered for efficient and selective bioconjugation, enabling the stable attachment of biomolecules under mild conditions. It is a critical tool for researchers and manufacturers in pharmaceutical development, proteomics, and advanced materials science, where precise and reliable chemical linkage is required.

Application

• Bioconjugation and Antibody-Drug Conjugate (ADC) Synthesis: The PFP ester reacts efficiently with primary amines, while the azide group enables subsequent click chemistry (e.g., CuAAC or SPAAC) for site-specific labeling and payload attachment.
• Proteomics and Biomarker Discovery: Used for labeling peptides, proteins, and other biomolecules with tags, fluorophores, or affinity handles for detection, purification, and analysis.
• Polymer and Materials Functionalization: Serves as a key intermediate for introducing azide functionality into polymers, surfaces, and nanomaterials for further modification via click chemistry.
• Chemical Biology Probe Development: Enables the creation of activity-based probes for studying enzyme function, protein-protein interactions, and cellular pathways.
• Diagnostic Reagent Manufacturing: Utilized in the production of diagnostic kits and assays where stable covalent linkages between detection molecules and substrates are essential.
• Solid-Phase Peptide Synthesis (SPPS): Acts as a versatile building block for introducing the azido-PEG spacer during the synthesis of modified peptides.

Basic Information

Product Name	Azido-Peg1-Pfp Ester
CAS No.	1807505-32-3
Molecular Formula	C₁₃H₁₁F₅N₄O₃
Molecular Weight	366.25 g/mol
Synonyms	1-Azido-2-(2-(2,3,4,5,6-pentafluorophenoxy)-2-oxoethoxy)ethane; 2-[2-(2-Azidoethoxy)ethoxy]-2-oxoethyl 2,3,4,5,6-pentafluorobenzoate; Azido-PEG1-PFP ester; Azido-PEG1-Pentafluorophenyl ester; PFP-PEG1-Azide; N3-PEG1-PFP ester; Pentafluorophenyl (2-(2-azidoethoxy)ethoxy)acetate
EINECS	Contact for details

Quality Control

Our Azido-Peg1-Pfp Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high chemical purity and functional integrity. Certificates of Analysis (COA) detailing identity, purity, and impurity profiles are provided with every shipment to support your research and quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry, cool place. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to prevent degradation. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	Colorless to pale yellow liquid or oil
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in DMSO, DMF, acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.