Description

Coumarin 343 Azide is a functionalized coumarin derivative designed for bioorthogonal labeling and advanced material science applications. This compound matters for its role as a critical click chemistry reagent, enabling precise covalent attachment of fluorescent coumarin tags to biomolecules and polymers under mild, aqueous conditions. It is essential for researchers and manufacturers in bioconjugation, chemical biology, and the development of fluorescent probes and sensors.

Application

• Bioorthogonal Labeling: Site-specific fluorescent tagging of proteins, antibodies, and nucleic acids via copper-free click chemistry (e.g., strain-promoted azide-alkyne cycloaddition, SPAAC).
• Chemical Biology Probes: Synthesis of activity-based probes (ABPs) and imaging agents for studying enzyme function and cellular processes.
• Polymer & Material Science: Functionalization of polymers, hydrogels, and nanoparticles to introduce fluorescent properties for tracking and characterization.
• Diagnostic Assay Development: Conjugation to detection molecules in lateral flow assays, ELISA, and other immunoassay platforms.
• Super-Resolution Microscopy: Creation of high-performance fluorescent dyes for techniques like STORM and PALM.
• Pharmaceutical Research: Labeling of drug candidates and delivery vehicles (e.g., liposomes, antibodies) for pharmacokinetic and biodistribution studies.

Basic Information

Item	Detail
Product Name	Coumarin 343 Azide
CAS No.	1807503-82-7
Molecular Formula	C19H15N5O5
Molecular Weight	393.36 g/mol
Synonyms	7-Diethylaminocoumarin-3-carboxylic Acid Azide; Coumarin 343 NHS Ester Azide derivative; 3-Azidocarbonyl-7-(diethylamino)coumarin; Azido-Coumarin 343; C343 Azide; 7-(Diethylamino)-2-oxo-2H-chromene-3-carboxylic acid azide; 3-Azido-7-diethylaminocoumarin
EINECS	Contact for details

Quality Control

Our Coumarin 343 Azide is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional testing for azide activity. Certificates of Analysis (COA) with detailed chromatograms and spectral data are provided to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant (e.g., in a sealed bag with silica gel) to protect from moisture. For long-term stability, store under inert atmosphere (argon or nitrogen). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 1.0 %
Functional Activity	≥ 90% active azide by click reaction assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.