Description

Cy3.5 Carboxylic Acids are a specialized class of cyanine dye derivatives featuring a carboxylic acid functional group, enabling covalent conjugation to biomolecules. This compound is essential for creating highly sensitive and stable fluorescent probes for advanced detection and imaging applications. It is primarily utilized by researchers and manufacturers in the life sciences, diagnostics, and pharmaceutical development sectors who require precise labeling of proteins, antibodies, and nucleic acids.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to antibodies, proteins, peptides, and oligonucleotides for use in immunoassays (ELISA, Western Blot) and flow cytometry.
• Bioimaging and Diagnostics: Synthesis of targeted imaging probes for in vitro and in vivo fluorescence microscopy and small animal imaging studies.
• Diagnostic Kit Development: Key component in the manufacturing of fluorescent lateral flow assays and other point-of-care diagnostic devices.
• Nucleic Acid Detection: Labeling of DNA and RNA probes for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) assays to label targets for fluorescence polarization (FP) and fluorescence resonance energy transfer (FRET) studies.
• Material Science: Functionalization of nanoparticles and polymers to create fluorescent materials for sensing and tracking applications.

Basic Information

Product Name	Cy3.5 Carboxylic Acids
CAS No.	1802928-88-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cyanine3.5 carboxylic acid; Sulfo-Cyanine3.5 acid; Cy3.5 NHS ester precursor; 1H-Benz[e]indolium, 2-[5-(1-carboxy-3-sulfopropyl)-1,3-butadienyl]-3-ethyl-1-(4-sulfobutyl)-, inner salt; Cy3.5™ carboxylic acid; Fluorescent dye Cy3.5 acid; Near-infrared cyanine dye acid derivative
EINECS	Contact for details

Quality Control

Our Cy3.5 Carboxylic Acids are produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and spectrophotometry to verify optical properties. Certificates of Analysis (COA) detailing lot-specific results are provided to ensure traceability and compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark green to purple solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	~591 nm
Emission Maximum (λem)	~604 nm
Extinction Coefficient (ε)	Contact for details
Moisture Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF, water (basic pH)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.