Description

Sulfo-Cy3-Methyltetrazine is a water-soluble, near-infrared fluorescent dye conjugate designed for bioorthogonal labeling applications. This compound is engineered for high-performance in demanding biological imaging and diagnostic assays, offering superior brightness and photostability. It is an essential reagent for researchers and developers in the fields of bioconjugation, in vivo imaging, and the development of antibody-drug conjugates (ADCs) and other targeted therapeutics.

Application

• Bioorthogonal Labeling: Used in inverse electron-demand Diels-Alder (IEDDA) reactions with trans-cyclooctene (TCO) or norbornene-modified biomolecules for highly specific, rapid, and catalyst-free conjugation.
• In Vivo Imaging: The sulfo-cyanine 3 (Cy3) fluorophore and near-infrared emission profile make it suitable for sensitive fluorescence imaging in live animal models.
• Antibody-Drug Conjugate (ADC) Development: Serves as a fluorescent payload or linker-payload model for tracking ADC distribution, pharmacokinetics, and target engagement.
• Flow Cytometry & Microscopy: Enables high-resolution cellular and subcellular imaging and analysis due to its bright fluorescence and good water solubility.
• Protein & Nucleic Acid Labeling: Facilitates the fluorescent tagging of proteins, peptides, and oligonucleotides for detection and quantification studies.
• Diagnostic Assay Development: Employed in the design of sensitive fluorescent immunoassays and molecular diagnostic probes.

Basic Information

Product Name	Sulfo-Cy3-Methyltetrazine
CAS No.	1801924-47-9
Molecular Formula	C41H45N7O8S2+ • (Counterion)-
Molecular Weight	~828.0 g/mol (free acid form, may vary with counterion)
Synonyms	Sulfo-Cyanine3-Methyltetrazine; Sulfo-Cy3-MeTz; Cy3-sulfo-methyltetrazine; 1-(6-((E)-2-((E)-3,3-Dimethyl-5-sulfo-1-(3-sulfopropyl)indolin-2-ylidene)ethylidene)-2-((E)-2-((E)-3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)-3H-indol-1-ium-2-yl)vinyl)-4-methyl-3,4-dihydropyridin-1(2H)-yl)ethan-1-one-2,5-dione; Methyltetrazine-Cy3 sulfonated; Water-soluble Cy3-tetrazine dye
EINECS	Contact for details

Quality Control

Our Sulfo-Cy3-Methyltetrazine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for life science research and diagnostic applications. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting critical quality attributes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	Dark green to purple solid
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Extinction Coefficient (ε)	~150,000 M-1cm-1 (at λmax)
Excitation/Emission Maxima	~550 nm / ~570 nm
Water Content (KF)	≤ 5.0%
Solubility	Freely soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.