Description

Sulfo-Cy5.5 Azide is a near-infrared (NIR) fluorescent dye derivative functionalized with a reactive azide group and water-solubilizing sulfonate moieties. This compound matters for researchers and developers requiring efficient, bio-orthogonal conjugation via click chemistry for labeling biomolecules in aqueous environments. Professionals in bioconjugation, in vivo imaging, diagnostic assay development, and flow cytometry need this high-performance reagent for creating stable, bright fluorescent probes with minimal background interference.

Application

• Bioconjugation and Probe Synthesis: Click chemistry labeling of proteins, antibodies, oligonucleotides, and other biomolecules with azide-reactive handles.
• In Vivo and In Vitro Imaging: Development of NIR fluorescent probes for deep-tissue imaging, tumor targeting, and cellular tracking due to reduced tissue autofluorescence.
• Diagnostic Assay Development: Fluorescent labeling for ELISA, lateral flow assays, and other immunoassays requiring high sensitivity in the NIR spectrum.
• Flow Cytometry and Cell Sorting: Staining of cell surface markers or intracellular targets for detection with NIR laser-equipped cytometers.
• Microscopy: Fluorescence microscopy applications, including super-resolution imaging, where photostability and brightness are critical.
• Material Science: Surface functionalization and labeling of nanoparticles, polymers, and hydrogels for tracking and characterization.

Basic Information

Product Name	Sulfo-Cy5.5 Azide
CAS No.	1801695-56-6
Molecular Formula	C44H49KN4O10S3
Molecular Weight	977.23 g/mol
Synonyms	Sulfo-Cyanine5.5 Azide; Sulfo-Cy5.5 N3; Cy5.5 Sulfonyl Azide; Sulfo-Cyanine 5.5 Azide Potassium Salt; Water-soluble Cy5.5 Azide; NIR Fluorescent Azide; 2-((1E,3E)-5-((E)-1-(5-azidopentyl)-3,3-dimethyl-5-sulfoindolin-2-ylidene)penta-1,3-dien-1-yl)-1-ethyl-3,3-dimethyl-3H-indol-1-ium-5-sulfonate
EINECS	Contact for details

Quality Control

Our Sulfo-Cy5.5 Azide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and spectrophotometry to validate optical properties. Certificates of Analysis (COA) detailing batch-specific results are available upon request, ensuring traceability and compliance with your research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Extinction Coefficient (ε)	≥ 190,000 cm-1M-1 at λmax
λAbsorption Max	~ 683 nm
λEmission Max	~ 703 nm
Water Content (KF)	≤ 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.