Description

5(6)-Cfda n-Succinimidyl Ester is a reactive fluorescent labeling reagent, specifically an amine-reactive succinimidyl ester derivative of the CFDA fluorophore. This compound is critical for the covalent labeling of proteins, peptides, and other biomolecules containing primary amines, enabling sensitive detection and analysis. It is primarily utilized by researchers and manufacturers in the fields of bioconjugation, proteomics, diagnostic assay development, and flow cytometry.

Application

• Protein and Antibody Labeling: Covalent conjugation to primary amine groups (e.g., lysine residues) for fluorescence-based detection.
• Bioconjugation Chemistry: A key intermediate for creating fluorescently tagged probes, sensors, and diagnostic reagents.
• Flow Cytometry & Microscopy: Preparation of fluorescently labeled antibodies and other cellular markers for cell analysis and imaging.
• Diagnostic Assay Development: Used in the manufacture of fluorescent immunoassays (e.g., ELISA, lateral flow assays).
• Proteomics Research: Labeling of peptides and proteins for gel electrophoresis, blotting, and other analytical techniques.
• Biomaterial and Nanoparticle Functionalization: Attaching fluorescent tags to surfaces for tracking and visualization studies.

Basic Information

Product Name	5(6)-Cfda n-Succinimidyl Ester
CAS No.	1794780-70-3
Molecular Formula	C25H15F3N2O7
Molecular Weight	512.40 g/mol
Synonyms	5(6)-CFDA, SE; CFDA N-hydroxysuccinimide ester; 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester; 5(6)-Carboxyfluorescein diacetate, succinimidyl ester; 5(6)-FAM, SE (diacetate); 5(6)-FAM diacetate, SE; CFDA-SE; 5(6)-Carboxyfluorescein Diacetate, N-Succinimidyl Ester
EINECS	Contact for details

Quality Control

Our 5(6)-Cfda n-Succinimidyl Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure high batch-to-batch consistency and performance reliability. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥95.0%
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	≤1.0%
Fluorescence Properties	Ex/Em ~492/517 nm (upon hydrolysis)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.