Description

Cy5 Azide is a near-infrared fluorescent labeling reagent designed for bioorthogonal click chemistry applications, specifically copper-free strain-promoted azide-alkyne cycloaddition (SPAAC). This compound is essential for researchers and developers requiring high-sensitivity, low-background labeling of biomolecules in complex biological systems without cytotoxic copper catalysts. It is a critical tool for professionals in biopharmaceutical R&D, in vivo imaging, diagnostic assay development, and advanced materials science.

Application

• Bioimaging Probes: Conjugation to cyclooctyne-modified antibodies, peptides, or small molecules for targeted in vitro and in vivo fluorescence imaging.
• Proteomics & Biomarker Discovery: Labeling of glycans, lipids, or other biomolecules via metabolic incorporation of azide-tagged precursors for gel-based or microarray analysis.
• Diagnostic Assay Development: Used as a fluorescent tag in lateral flow assays (LFAs), ELISA, or other immunoassays to enhance detection limits.
• Polymer & Materials Science: Functionalization of nanoparticles, hydrogels, or surfaces with azide groups for fluorescence tracking and characterization.
• Cell Biology Studies: Real-time tracking of cell surface dynamics, receptor internalization, and protein trafficking.
• Drug Delivery & Pharmacokinetics: Labeling of drug conjugates or nanocarriers to monitor distribution, uptake, and clearance in preclinical models.

Basic Information

Item	Details
Product Name	Cy5 Azide
CAS No.	1782950-80-4
Molecular Formula	C37H45N6O3+
Molecular Weight	621.80 g/mol
Synonyms	CY5 azide; Sulfo-Cyanine5 azide; Cyanine5 N3; 1-(5-Carboxypentyl)-2-((1E,3E)-5-(1-(6-azidohexyl)-3,3-dimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-3,3-dimethyl-3H-indol-1-ium; NIR fluorescent azide; SPAAC dye; Click chemistry dye 647 nm
EINECS	Contact for details

Quality Control

Our Cy5 Azide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research and development protocols.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This compound is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark blue to purple solid or film
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular ion [M]+
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Extinction Coefficient	≥ 250,000 L·mol-1·cm-1 (≈647 nm in methanol)
Fluorescence Emission	≈ 670 nm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.