Description

Cy3 DBCO (CAS NO 1782950-79-1) is a high-purity, functionalized cyanine dye derivative designed for advanced bioorthogonal conjugation. This compound is engineered for efficient, copper-free click chemistry, enabling rapid and specific labeling of biomolecules in complex biological systems without interfering with native biochemical processes. It is an essential reagent for researchers and developers in bioconjugation, pharmaceutical development, and diagnostic assay creation, where precise and stable fluorescent tagging is required.

Application

• Bioorthogonal Labeling: Site-specific fluorescent labeling of antibodies, proteins, peptides, and oligonucleotides via strain-promoted azide-alkyne cycloaddition (SPAAC).
• Live-Cell Imaging: Real-time tracking and visualization of cellular processes, receptor trafficking, and biomolecule localization using its near-infrared fluorescence.
• Diagnostic Probe Development: Construction of sensitive fluorescent probes and biosensors for in vitro diagnostics (IVD) and high-throughput screening (HTS) assays.
• Drug Delivery & Targeting: Conjugation to drug carriers, nanoparticles, and targeting ligands for theranostic applications to monitor drug distribution.
• Flow Cytometry & Microscopy: Used as a vital stain for multicolor panel design in flow cytometry and super-resolution microscopy studies.
• Material Science Functionalization: Surface modification of polymers, hydrogels, and nanomaterials to introduce fluorescent properties for tracking and analysis.

Basic Information

Product Name	Cy3 DBCO
CAS No.	1782950-79-1
Molecular Formula	C46H48N4O3+
Molecular Weight	704.91 g/mol
Synonyms	DBCO-Cy3; Cyanine3 DBCO; Dibenzocyclooctyne-Cy3; 1-(6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethyl-2-((E)-3-((E)-1-(6-(3-(3a,6a-Dihydro-1H-pyrrolo[3,4-c]pyrazol-5(4H,6H)-yl)propyl)-3,3-dimethyl-5-sulfo-3H-indol-1-ium-2-yl)prop-2-en-1-ylidene)-2-chlorocyclopent-1-en-1-yl)-5-sulfo-1H-indol-1-ium; Sulfo-Cy3 DBCO; DBCO-sulfo-Cy3; DBCO-Cy3 NHS ester (related precursor)
EINECS	Contact for details

Quality Control

Our Cy3 DBCO is manufactured under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and spectrophotometry for functional performance. Certificates of Analysis (COA) detailing purity, identity, and performance data are available upon request to ensure compliance with your research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Extinction Coefficient (ε)	~150,000 cm-1M-1 at λmax
Solubility	Soluble in DMSO, DMF, water (sulfonated form)
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.