Description

Cyanine7.5 Carboxylic Acid CAS NO 1686147-68-1 is a near-infrared (NIR) fluorescent dye derivative functionalized with a carboxylic acid group, enabling covalent conjugation to biomolecules. This compound is critical for developing highly sensitive diagnostic assays and advanced bioimaging probes due to its deep tissue penetration and minimal background autofluorescence. It is primarily needed by researchers and manufacturers in the biotechnology, pharmaceutical, and in-vitro diagnostics (IVD) sectors for applications in fluorescence microscopy, flow cytometry, and in vivo imaging.

Application

• Bioconjugation and Probe Development: The carboxylic acid group allows for covalent attachment to antibodies, peptides, proteins, and oligonucleotides, creating targeted fluorescent probes.
• In Vivo Imaging: Utilized in preclinical research for non-invasive deep-tissue imaging in small animal models, leveraging its NIR emission window.
• Flow Cytometry and Cell Sorting: Serves as a fluorescent tag for cell surface markers and intracellular targets in multiparameter analysis.
• Fluorescence Microscopy: Used for high-resolution cellular and subcellular imaging with reduced phototoxicity compared to visible light dyes.
• Diagnostic Assay Development: A key component in designing sensitive lateral flow assays (LFAs), ELISA, and other immunoassays for point-of-care and clinical diagnostics.
• Drug Delivery Tracking: Conjugated to nanoparticles or therapeutic agents to monitor biodistribution, pharmacokinetics, and target engagement.

Basic Information

Product Name	Cyanine7.5 Carboxylic Acid
CAS No.	1686147-68-1
Molecular Formula	C44H51N3O2S2+ (as a common counterion form)
Molecular Weight	~717.9 g/mol (Free acid form)
Synonyms	Cy7.5 Carboxylic Acid; Sulfo-Cyanine7.5 carboxylic acid (common trade form); NIR-797 Carboxylic Acid; Cyanine 7.5 COOH; 2-((1E,3E)-5-((E)-2-((1E,3E)-3-(2-Carboxyethyl)-5-chloro-1-(5-sulfonatopentyl)-3-(sulfonatopropyl)indol-1-ium-2-yl)vinyl)-4-((E)-2-((E)-3-(2-carboxyethyl)-5-chloro-1-(5-sulfonatopentyl)-1,3-dihydro-3H-indol-2-ylidene)ethylidene)-1-cyclopenten-1-yl)vinyl)-1-(5-sulfonatopentyl)-5-chloro-3-(sulfonatopropyl)-3H-indol-1-ium; IR-786 Carboxylic Acid derivative; Heptamethine cyanine dye carboxylic acid.
EINECS	Contact for details

Quality Control

Our Cyanine7.5 Carboxylic Acid is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and spectrophotometry to verify optical properties. Certificates of Analysis (COA) detailing purity, absorbance/emission maxima, and extinction coefficient are provided to ensure compliance with research and diagnostic application standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	~788 ± 5 nm
Emission Maximum	~808 ± 5 nm
Extinction Coefficient (ε)	≥ 200,000 M-1cm-1
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.