Description

Sulfo-Cyanine3 Azide Methyl is a water-soluble, near-infrared fluorescent dye derivative designed for bioorthogonal labeling via copper-free click chemistry. This compound matters for researchers and developers requiring high-sensitivity, low-background conjugation of biomolecules in aqueous environments, particularly for live-cell imaging and *in vivo* applications. It is essential for professionals in bioconjugation, pharmaceutical R&D, diagnostic assay development, and advanced materials science who need reliable, bright, and stable fluorescent probes.

Application

• Bioconjugation and Probe Labeling: Site-specific labeling of proteins, antibodies, oligonucleotides, and other biomolecules via strain-promoted azide-alkyne cycloaddition (SPAAC).
• Live-Cell and *In Vivo* Imaging: Fluorescent tagging of cellular components for real-time tracking and visualization in biological systems with minimal cytotoxicity.
• Diagnostic Assay Development: Used as a critical component in developing high-sensitivity fluorescence-based diagnostic kits and biosensors.
• Flow Cytometry and Microscopy: Enables multicolor analysis and high-resolution imaging in cytometry and fluorescence microscopy applications.
• Polymer and Materials Science: Functionalization of nanoparticles, hydrogels, and other polymeric materials with fluorescent properties for tracking and analysis.
• Drug Delivery Research: Labeling of drug carriers, liposomes, and other delivery vehicles to study biodistribution and pharmacokinetics.

Basic Information

Product Name	Sulfo-Cyanine3 Azide Methyl
CAS No.	1658416-54-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Sulfo-Cy3 Azide Methyl ester; Sulfo Cy3 N3; Cyanine3 sulfonated azide; Water-soluble Cy3 azide; Sulfo-Cyanine 3 azide methyl ester; Near-IR fluorescent azide dye; SPAAC dye Sulfo-Cy3; Bioorthogonal labeling dye 1658416-54-6
EINECS	Contact for details

Quality Control

Our Sulfo-Cyanine3 Azide Methyl is produced under stringent quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification, to ensure it meets the high standards required for sensitive life science applications. Certificates of Analysis (COA) with detailed chromatographic and spectroscopic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a dry environment before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark green to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Extinction Coefficient	≥ 150,000 cm⁻¹M⁻¹
Fluorescence Emission	~570 nm
Solubility	Soluble in water, DMSO, DMF
Moisture Content (KF)	< 5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.