Description

Icg-Nhs(Mono-Sulfo-Cy7.5 Nhs) is a near-infrared (NIR) fluorescent dye derivative, specifically a water-soluble, sulfonated cyanine 7.5 dye activated with an N-hydroxysuccinimide (NHS) ester for efficient biomolecule conjugation. This compound is critical for creating highly sensitive and stable fluorescent probes with minimal background interference in the NIR window. It is primarily needed by researchers and manufacturers in bioconjugation, in vivo imaging, flow cytometry, and diagnostic assay development.

Application

• Antibody and Protein Labeling: Conjugation to monoclonal antibodies, proteins, and peptides for use in immunofluorescence, Western blotting, and immunohistochemistry.
• In Vivo Imaging Probes: Creation of targeted imaging agents for deep-tissue fluorescence imaging in preclinical small animal studies.
• Nucleic Acid Labeling: Labeling of oligonucleotides, DNA, and RNA for fluorescence in situ hybridization (FISH) and real-time PCR detection.
• Flow Cytometry & Cell Sorting: Development of fluorescent markers for cell surface receptor staining and high-throughput cell analysis.
• Diagnostic Kit Development: Integration into lateral flow assays (LFAs) and other point-of-care diagnostic devices for sensitive detection.
• Polymer and Nanoparticle Functionalization: Covalent attachment to polymers, dendrimers, and nanoparticles to create advanced drug delivery or imaging systems.

Basic Information

Product Name	Icg-Nhs(Mono-Sulfo-Cy7.5 Nhs)
CAS No.	1622335-40-3
Molecular Formula	C52H61KN4O13S2
Molecular Weight	~1061.36 g/mol (Potassium salt form)
Synonyms	Sulfo-Cyanine7.5 NHS ester; Mono-sulfo Cy7.5 NHS ester; Cy7.5 NHS ester, sulfonated; ICG derivative NHS ester; 1-(6-((E)-2-((E)-2-(1-(5-Carboxypentyl)-3,3-dimethyl-5-sulfoindolin-2-ylidene)ethylidene)-3-((E)-2-(1-(5-carboxypentyl)-3,3-dimethylindolin-2-ylidene)ethylidene)cyclohex-1-en-1-yl)vinyl)-1,3,3-trimethyl-3H-indol-1-ium-5-sulfonate NHS ester potassium salt; NIR dye NHS ester; Water-soluble Cy7.5 NHS
EINECS	Contact for details

Quality Control

Our Icg-Nhs(Mono-Sulfo-Cy7.5 Nhs) is manufactured under strict quality protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and spectrophotometric analysis to ensure defined absorbance/emission profiles. We provide comprehensive Certificates of Analysis (COA) detailing all specifications, ensuring traceability and compliance with research-grade material standards.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis of the active NHS ester.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Absorption (λmax)	788 ± 5 nm
Emission (λmax)	808 ± 5 nm
Extinction Coefficient (ε)	≥ 200,000 cm⁻¹M⁻¹
Water Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.