Description

Cy5-N3 is a near-infrared fluorescent dye functionalized with an azide group, enabling highly specific bioorthogonal labeling via click chemistry. This compound is essential for researchers and developers requiring precise, covalent tagging of biomolecules in complex biological systems without interfering with native biochemical processes. It is primarily used in the life sciences and pharmaceutical industries for applications such as fluorescence imaging, proteomics, and the development of diagnostic assays and targeted therapeutics.

Application

• Bioorthogonal Labeling: Covalent conjugation to alkyne-modified proteins, antibodies, nucleic acids, or small molecules for fluorescence detection.
• Live-Cell Imaging: Real-time tracking and visualization of cellular processes, receptor trafficking, and biomolecule localization using its near-infrared emission.
• Proteomics & Biomarker Discovery: Tagging and enrichment of specific protein targets for analysis via gel electrophoresis or mass spectrometry.
• Diagnostic Assay Development: Creating fluorescent probes and conjugates for in vitro diagnostics (IVD), including lateral flow and ELISA-based tests.
• Drug Delivery & Targeting: Conjugation to drug carriers, nanoparticles, or targeting ligands to monitor distribution and efficacy in preclinical studies.
• Flow Cytometry: Fluorescent labeling of cell surface markers or intracellular targets for high-throughput cell analysis and sorting.
• Materials Science: Functionalization of polymers, surfaces, or nanomaterials with fluorescent properties for sensing applications.

Basic Information

Product Name	Cy5-N3
CAS No.	1621101-43-6
Molecular Formula	C37H45N6O3+
Molecular Weight	621.80 g/mol
Synonyms	Cyanine5 azide; Sulfo-Cy5 azide (common variant); Cy5® N3; 1-(5-Carboxypentyl)-2-((1E,3E)-5-((E)-1-(6-azidohexyl)-3,3-dimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-3,3-dimethyl-3H-indol-1-ium; Azide-functionalized Cy5 dye; NIR azide dye; Click chemistry Cy5 dye; Cy5 alkyne reaction partner
EINECS	Contact for details

Quality Control

Our Cy5-N3 is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. Certificates of Analysis (COA) detailing purity, identity, and performance data are provided with every shipment to ensure compliance with research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep the material under an inert atmosphere. Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with molecular structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Extinction Coefficient	≥ 250,000 cm-1M-1 at λmax
Functional Group Integrity	Azide content confirmed by FT-IR or reaction yield

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.