Description

Azide-Ss-Biotin is a heterobifunctional crosslinking reagent designed for bioconjugation, featuring an azide group for click chemistry and a biotin moiety for affinity purification or detection. This compound is essential for researchers and developers in the life sciences who require precise, stable, and efficient labeling of biomolecules. It is particularly valuable for applications in proteomics, antibody-drug conjugate (ADC) development, and the creation of diagnostic assays where high specificity and sensitivity are paramount.

Application

• Bioconjugation and Labeling: Used for site-specific conjugation of biomolecules (e.g., proteins, antibodies, oligonucleotides) via copper-free click chemistry with alkynes.
• Proteomics Research: Enables the enrichment and isolation of target proteins or protein complexes through the strong biotin-streptavidin interaction for mass spectrometry analysis.
• Antibody-Drug Conjugate (ADC) Development: Serves as a linker-payload component in the synthesis of novel ADCs, facilitating the attachment of cytotoxic agents to monoclonal antibodies.
• Diagnostic Assay Development: Utilized in the fabrication of biosensors and immunoassays (e.g., ELISA) for the detection and quantification of analytes.
• Cell Surface Engineering: Applied for labeling and tracking cell surface receptors or glycans in flow cytometry and fluorescence microscopy.
• Material Science Functionalization: Used to introduce biotin tags onto nanoparticles, polymers, or surfaces for subsequent biomolecular capture and analysis.
• Chemical Biology Probes: Acts as a key reagent in the synthesis of activity-based probes for studying enzyme function and metabolic pathways.

Basic Information

Product Name	Azide-Ss-Biotin
CAS No.	1620523-64-9
Molecular Formula	C19H33N7O4S3
Molecular Weight	519.71 g/mol
Synonyms	Biotin-PEG3-SS-Azide; Biotin-PEG3-SS-N3; Biotin-(PEG)3-SS-Azide; 5-((3-(2-(2-(3-Azidopropoxy)ethoxy)ethoxy)propyl)disulfanyl)pentanoic acid N-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoyl)amide; Biotin-PEG3-SS-N3; Azido-PEG3-SS-Biotin; N3-PEG3-SS-Biotin; SS-Biotin-Azide
EINECS	Contact for details

Quality Control

Our Azide-Ss-Biotin is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and NMR/MS for structural confirmation, to ensure high batch-to-batch consistency and compliance with research-grade specifications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.