Description

Alexa Fluor 647 NHS Ester is a high-performance, near-infrared fluorescent labeling reagent designed for the covalent conjugation of proteins, antibodies, and other amine-containing biomolecules. This compound is critical for researchers and developers requiring stable, bright, and photostable conjugates for multiplexed detection and advanced imaging applications. It is essential for professionals in biotechnology, pharmaceutical R&D, and diagnostic assay development who rely on precise and reliable fluorescent probes.

Application

• Antibody Labeling: Conjugation to monoclonal and polyclonal antibodies for flow cytometry, immunofluorescence (IF), and immunohistochemistry (IHC).
• Protein & Peptide Tagging: Fluorescent labeling of proteins, enzymes, and peptides for fluorescence resonance energy transfer (FRET) and binding studies.
• Nucleic Acid Detection Probes: Preparation of labeled oligonucleotides and nucleotides for fluorescence in situ hybridization (FISH) and microarray analysis.
• Diagnostic Assay Development: Manufacturing key components for lateral flow assays, ELISA, and other clinical diagnostic kits.
• Live-Cell & Super-Resolution Imaging: Creating stable probes for long-term live-cell tracking and high-resolution microscopy techniques like STORM and PALM.
• Bioconjugation & Chemical Biology: A versatile tool for site-specific labeling in bioconjugation strategies and probe development.

Basic Information

Product Name	Alexa Fluor 647 NHS Ester
CAS No.	1620475-28-6
Molecular Formula	C45H49N4O14S33- • 3 Na+ (or similar, salt form dependent)
Molecular Weight	~1250 g/mol (Contact for exact salt form details)
Synonyms	AF647 NHS Ester; Alexa Fluor® 647 Succinimidyl Ester; Alexa647-NHS; 6-((6-((((2,5-Dioxopyrrolidin-1-yl)oxy)carbonyl)amino)hexanoyl)amino)-6-oxohexyl)triphenyl-λ5-azanyl; Alexa Fluor 647 carboxylic acid succinimidyl ester; Succinimidyl ester of Alexa Fluor 647; AF647-SE; Alexa Fluor® 647 C2 maleimide (common related compound, often searched together)
EINECS	Contact for details

Quality Control

Our Alexa Fluor 647 NHS Ester is manufactured under strict quality systems to ensure batch-to-batch consistency and performance. Each lot is characterized by advanced analytical techniques including HPLC, mass spectrometry, and NMR to confirm identity, purity, and labeling efficiency. Certificates of Analysis (COA) with detailed spectral data are provided to support your regulatory and quality documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Dark blue to purple solid or film
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 90% (Dye content)
Absorption (λmax)	650 ± 3 nm
Emission (λmax)	668 ± 3 nm
Extinction Coefficient (ε)	~270,000 cm-1M-1
Degree of Labeling (DOL) Check	Conforms (via control conjugation)
Solubility	Soluble in DMSO, DMF, and other anhydrous polar aprotic solvents
Water Content (KF)	< 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.