Description

Disulfo-Cy5 Alkyne is a functionalized cyanine dye derivative designed for bioorthogonal labeling via copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry. Its core value lies in combining the bright, far-red fluorescence of the Cy5 chromophore with enhanced water solubility and a reactive alkyne handle, enabling efficient, site-specific conjugation to azide-modified biomolecules. This product is essential for researchers and developers in bioconjugation, proteomics, bioimaging, and diagnostic assay development, where stable, fluorescent labeling of proteins, antibodies, and oligonucleotides is required.

Application

• Fluorescent Labeling of Biomolecules: Site-specific conjugation to azide-tagged proteins, antibodies, peptides, and nucleic acids for creating stable fluorescent probes.
• Live-Cell and In Vivo Imaging: Utilization in CuAAC click chemistry protocols for labeling cellular components in live systems due to its far-red emission, which minimizes autofluorescence and increases tissue penetration.
• Proteomics and Biomarker Discovery: Tagging of azide-functionalized metabolites or glycans for visualization and analysis in gel-based or microarray platforms.
• Diagnostic Assay Development: Conjugation to detection antibodies or oligonucleotides for use in fluorescence-based immunoassays (e.g., ELISA, Western blotting) or in situ hybridization (FISH).
• Material Science and Polymer Chemistry: Fluorescent labeling of azide-functionalized polymers, nanoparticles, or surfaces to track material distribution and interaction.
• Flow Cytometry and Cell Sorting: Preparation of fluorescent antibody conjugates or other cell surface markers for high-throughput cell analysis and sorting.

Basic Information

Product Name	Disulfo-Cy5 Alkyne
CAS No.	1617497-19-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Sulfo-Cyanine5 alkyne; Cy5® alkyne, disulfonated; 1,1'-(4,4,7,7-Tetramethyl-4,7-dihydro-1H-cyclopenta[b]quinoxaline-2,5-diyl)bis(ethyne-2,1-diyl))bis(N,N,N-trimethyl-5-sulfopentan-1-aminium) inner salt, alkyne derivative; Water-soluble Cy5 alkyne; Bis-sulfonated Cy5 alkyne; Far-red fluorescent alkyne dye
EINECS	Contact for details

Quality Control

Our Disulfo-Cy5 Alkyne is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and spectrophotometry for absorbance and fluorescence verification, to ensure consistency and performance for critical research applications. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This compound is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark blue to purple solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥95%
Absorption Maximum (λmax)	~649 nm
Emission Maximum (λem)	~670 nm
Extinction Coefficient (ε)	Contact for details
Solubility	Soluble in water, DMSO, DMF
Moisture Content (KF)	≤5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.