Description

Azido-PEG12-NHS ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an amine-reactive N-hydroxysuccinimide (NHS) ester at one terminus and a click chemistry-ready azide group at the other. This compound is essential for creating stable, hydrophilic spacers in bioconjugation, enabling the precise attachment of biomolecules, probes, or surfaces under mild aqueous conditions. It is a critical reagent for researchers and manufacturers in biopharmaceutical development, proteomics, and advanced materials science, facilitating the creation of antibody-drug conjugates (ADCs), diagnostic assays, and functionalized nanoparticles.

Application

• Bioconjugation and ADC Development: Used to link antibodies, proteins, or peptides to drugs, toxins, or fluorescent dyes via a hydrophilic PEG spacer to improve solubility and reduce immunogenicity.
• Surface Functionalization: For modifying nanoparticles, microspheres, biosensor chips, or other solid supports with azide groups for subsequent click chemistry coupling.
• Proteomics and Biomarker Discovery: Employed in labeling strategies for mass spectrometry, pull-down assays, and activity-based protein profiling (ABPP).
• Polymer and Hydrogel Synthesis: Acts as a crosslinking agent in the development of smart biomaterials, drug delivery systems, and tissue engineering scaffolds.
• Diagnostic Reagent Preparation: Conjugation of enzymes (e.g., HRP) or other reporter molecules to detection antibodies for ELISA and lateral flow assays.
• Chemical Biology Tool Development: Synthesis of probes for studying cellular processes, including metabolic labeling and imaging.

Basic Information

Product Name	Azido-PEG12-NHS Ester
CAS No.	1610796-02-5
Molecular Formula	C33H60N4O16
Molecular Weight	768.85 g/mol
Synonyms	NHS-PEG12-Azide; Azido-PEG12-NHS ester; N3-PEG12-NHS; α-Azido-ω-NHS ester PEG; 2,5-Dioxopyrrolidin-1-yl 3,6,9,12,15,18,21,24,27,30,33,36-dodecaoxaoctatetracontan-48-azide; PEG12 spacer crosslinker; Azido-PEG-NHS, 12 unit; Amine-to-Azide Heterobifunctional PEG Linker
EINECS	Contact for details

Quality Control

Our Azido-PEG12-NHS ester is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and functional group analysis. Certificates of Analysis (COA) detailing all specifications are provided with every shipment. We adhere to cGMP principles where applicable to support our customers in research, diagnostic, and pre-clinical development.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Assay (NHS Ester)	≥ 90%
Functional Assay (Azide)	≥ 90%
Solubility	Soluble in DMF, DMSO, acetonitrile; partially soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.