Description

Cyanine5.5 Maleimide is a near-infrared fluorescent dye derivative functionalized with a maleimide group for site-specific bioconjugation. This compound is essential for creating stable, fluorescently labeled probes for advanced bioimaging and diagnostic applications. It is primarily used by researchers and manufacturers in the fields of biopharmaceutical development, in vivo imaging, and high-sensitivity molecular diagnostics.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation to engineered cysteine residues on antibodies for targeted therapeutics.
• Protein & Peptide Labeling: Covalent attachment to thiol groups (-SH) on proteins, peptides, and enzymes for fluorescence detection.
• In Vivo & Whole-Animal Imaging: Creation of near-infrared (NIR) probes for deep-tissue imaging with minimal background autofluorescence.
• Flow Cytometry & Cell Sorting: Labeling of cell surface proteins or intracellular targets for multiplexed analysis.
• Diagnostic Assay Development: Used as a fluorescent reporter in ELISA, lateral flow assays, and other immunoassays.
• Nanoparticle Functionalization: Conjugation to thiolated nanoparticles for targeted imaging and drug delivery systems.
• DNA/RNA Labeling: Modification of thiol-modified oligonucleotides for fluorescence in situ hybridization (FISH) and other genomic techniques.
• Biomarker Detection Probes: Development of sensitive probes for the detection of specific biomarkers in research and clinical samples.

Basic Information

Product Name	Cyanine5.5 Maleimide
CAS No.	1593644-50-8
Molecular Formula	C44H49N3O5S2+ (as a common counterion form)
Molecular Weight	Contact for details
Synonyms	Cy5.5 Maleimide; Sulfo-Cyanine5.5 Maleimide (common variant); Cy™5.5 Maleimide; NIR dye maleimide; 1H-Pyrrole-2,5-dione, 1-[(1E,3E)-5-[2-[2-[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethoxy]ethoxy]ethoxy]-1,3-pentadien-1-yl]-3,3-dimethyl-2-[(1E,3E)-5-(1,3-dihydro-3,3-dimethyl-1-propyl-2H-indol-2-ylidene)-1,3-pentadien-1-yl]-3H-indolium; Near-infrared fluorescent maleimide dye
EINECS	Contact for details

Quality Control

Our Cyanine5.5 Maleimide is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via mass spectrometry and NMR, to ensure it meets the high standards required for life science research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance, fluorescence, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption Maximum (λmax)	~680 - 690 nm
Emission Maximum (λem)	~705 - 715 nm
Extinction Coefficient (ε)	Contact for details
Solubility	Soluble in DMSO, DMF, methanol
Water Content (KF)	< 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.