Description

4-o-[2-(2,5-Dioxopyrrolidin-1-Yl)Ethyl] 1-o-Methyl (E)-But-2-Enedioate CAS NO 1577222-14-0 is a specialized bifunctional chemical intermediate featuring both a maleimide ester and an activated NHS ester group. This compound is engineered for high-efficiency conjugation and cross-linking applications, enabling the stable attachment of molecules under mild conditions. It is primarily utilized by researchers and manufacturers in the pharmaceutical, bioconjugation, and advanced materials sectors for developing targeted drug delivery systems, diagnostic reagents, and functional polymers.

Application

• Bioconjugation Reagent: For the site-specific labeling of proteins, antibodies, peptides, and oligonucleotides via amine or thiol groups.
• Pharmaceutical Intermediate: In the synthesis of antibody-drug conjugates (ADCs) and other targeted therapeutic agents.
• Polymer Modification: For cross-linking or functionalizing polymers and hydrogels to introduce bioactive moieties.
• Diagnostic Probe Synthesis: Creating fluorescent or affinity-labeled probes for imaging and assay development.
• Surface Immobilization: Covalent attachment of biomolecules to sensor chips, nanoparticles, or other solid supports.
• Materials Science: Development of smart materials and coatings with tailored chemical reactivity.

Basic Information

Product Name	4-o-[2-(2,5-Dioxopyrrolidin-1-Yl)Ethyl] 1-o-Methyl (E)-But-2-Enedioate
CAS No.	1577222-14-0
Molecular Formula	C11H12N2O7
Molecular Weight	284.22 g/mol
Synonyms	1-o-Methyl 4-o-[2-(2,5-dioxo-1-pyrrolidinyl)ethyl] (2E)-2-butenedioate; Maleimide-PEG1-NHS Ester; NHS-PEG1-Maleimide; (E)-4-((2-(2,5-dioxopyrrolidin-1-yl)ethoxy)carbonyl)but-2-enoic acid methyl ester; Methyl (E)-4-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethoxy)carbonyl)but-2-enoate; PEG1 Maleimide NHS Ester; 1-(2-((4-Methoxy-4-oxobut-2-enoyl)oxy)ethyl)-1H-pyrrole-2,5-dione
EINECS	Contact for details

Quality Control

Every batch of 4-o-[2-(2,5-Dioxopyrrolidin-1-Yl)Ethyl] 1-o-Methyl (E)-But-2-Enedioate is manufactured under strict quality management systems and undergoes rigorous analytical testing. We ensure compliance with high-purity standards suitable for research and pharmaceutical development. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with each shipment to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at temperatures between -20°C to -10°C for long-term stability. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to prevent degradation. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.