Description

Cy5 Dbco CAS NO 1564286-24-3 is a high-purity, functionalized cyanine dye derivative designed for advanced bioconjugation and labeling applications. This compound is engineered for efficient, copper-free click chemistry, enabling rapid and specific covalent attachment to azide-modified biomolecules under mild, biocompatible conditions. It is an essential reagent for researchers and manufacturers in the fields of bioimaging, diagnostics, and pharmaceutical development, where precise and stable fluorescent labeling is critical.

Application

• Bioimaging Probes: Fluorescent labeling of antibodies, proteins, peptides, and oligonucleotides for in vitro and in vivo imaging studies.
• Diagnostic Assays: Development of fluorescent lateral flow assays (LFAs), ELISA, and other immunoassay platforms requiring high-sensitivity detection.
• Flow Cytometry: Conjugation to cell surface markers or intracellular targets for high-throughput cell analysis and sorting.
• Drug Delivery & Targeting: Labeling of nanoparticles, liposomes, or antibody-drug conjugates (ADCs) to track biodistribution and cellular uptake.
• Materials Science: Functionalization of polymers, surfaces, and hydrogels with near-infrared (NIR) fluorescent properties for sensor development.
• Chemical Biology: A key tool for studying biomolecular interactions, localization, and dynamics via click chemistry-mediated tagging.

Basic Information

Product Name	Cy5 Dbco
CAS No.	1564286-24-3
Molecular Formula	C45H49N4O3+
Molecular Weight	693.91 g/mol
Synonyms	DBCO-Cy5; Cyanine5 DBCO; DBCO-Cyanine5; Sulfo-Cy5 DBCO (related sulfonated form); 1,3-Diphenylisobenzofuran-5-yl DBCO-Cy5 conjugate; NIR DBCO dye; Dibenzocyclooctyne-Cy5; Azadibenzocyclooctyne-Cy5
EINECS	Contact for details

Quality Control

Every batch of Cy5 Dbco is manufactured under strict quality management protocols. Our products undergo rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to ensure high chemical purity (>95% typical) and consistent performance. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided with each shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Dark blue to purple solid or film
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Extinction Coefficient	~250,000 M-1cm-1 (at ~649 nm in methanol)
Emission Maximum	~670 nm
Solubility	Soluble in DMSO, DMF, methanol; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.