Description

Fluorescein-Peg4-Azide is a high-purity, multifunctional chemical probe designed for advanced bioconjugation and labeling applications. Its core value lies in combining the bright green fluorescence of fluorescein with a flexible tetraethylene glycol (PEG4) spacer and a bioorthogonal azide group, enabling highly specific, copper-free click chemistry reactions. This compound is essential for researchers and developers in pharmaceutical R&D, diagnostic assay development, and advanced materials science who require precise, stable, and efficient fluorescent tagging of biomolecules and surfaces.

Application

• Bioconjugation and Protein Labeling: Site-specific fluorescent labeling of antibodies, proteins, and peptides via strain-promoted azide-alkyne cycloaddition (SPAAC).
• Diagnostic Probe Development: Synthesis of fluorescent probes for in vitro diagnostics (IVD), fluorescence in situ hybridization (FISH), and cellular imaging assays.
• Polymer and Materials Functionalization: Incorporation into hydrogels, nanoparticles, and polymer backbones to introduce fluorescence and reactive handles for further modification.
• Cell Surface Engineering: Labeling of cell surface glycans or other metabolically incorporated azide-tagged biomolecules for flow cytometry and microscopy.
• Drug Delivery System Tracking: Fluorescent tagging of drug carriers, such as liposomes or dendrimers, to monitor distribution and uptake in preclinical studies.
• Chemical Biology Tool: Used in activity-based protein profiling (ABPP) and other discovery platforms to visualize molecular interactions.

Basic Information

Product Name	Fluorescein-Peg4-Azide
CAS No.	1454662-54-4
Molecular Formula	C37H43N5O11
Molecular Weight	733.77 g/mol
Synonyms	5-FAM-PEG4-Azide; 5-Carboxyfluorescein-PEG4-Azide; 6-FAM-PEG4-N3; Fluorescein-5-PEG4-Azide; 5(6)-Carboxyfluorescein Tetraethyleneglycol Azide; Azido-PEG4-5-FAM; Azido-PEG4-carboxyfluorescein; N3-PEG4-Fluorescein
EINECS	Contact for details

Quality Control

Every batch of Fluorescein-Peg4-Azide is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We ensure high batch-to-batch consistency with purity typically exceeding 95% (by HPLC). Our standard quality commitment includes verification of structure (NMR, MS), functional group integrity, and fluorescence properties. Certificates of Analysis (COA) detailing all specifications are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. Under these conditions, the product is stable for at least 24 months. The material is light-sensitive (store away from light). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	Orange to red solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption (λmax)	~492 nm
Emission (λmax)	~518 nm
Solubility	DMSO, DMF, Acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.