Description

Mal-PEG2-NHS Ester is a heterobifunctional crosslinking reagent featuring a maleimide group and an N-hydroxysuccinimide (NHS) ester, connected by a polyethylene glycol (PEG) spacer. This compound is specifically engineered for the stable, site-specific conjugation of biomolecules, such as antibodies or peptides, to other functional entities like drugs, dyes, or surfaces. It is an essential tool for researchers and manufacturers in bioconjugation, pharmaceutical development, and diagnostic assay creation, where controlled and reproducible linking is critical.

Application

• Antibody-Drug Conjugate (ADC) Synthesis: For the controlled attachment of cytotoxic payloads to monoclonal antibodies via thiol-maleimide chemistry.
• Protein and Peptide Labeling: Conjugation of fluorescent dyes, biotin, or other probes to cysteine-containing proteins for imaging and detection.
• Surface Functionalization: Immobilization of biomolecules onto nanoparticles, biosensors, or microarray slides to create bioactive surfaces.
• PEGylation Strategies: Introducing a short, hydrophilic PEG spacer to improve the solubility and pharmacokinetic properties of therapeutic biomolecules.
• Diagnostic Reagent Development: Creating stable conjugates for ELISA, lateral flow assays, and other immunoassay formats.
• Biomaterial Engineering: Crosslinking polymers or hydrogels for tissue engineering and drug delivery systems.

Basic Information

Product Name	Mal-PEG2-NHS Ester
CAS No.	1433997-01-3
Molecular Formula	C19H26N2O9
Molecular Weight	426.42 g/mol
Synonyms	Maleimide-PEG2-NHS Ester; NHS-PEG2-Maleimide; 3-((2-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethoxy)ethoxy)propanoic Acid 2,5-Dioxopyrrolidin-1-yl Ester; 1-[2-[2-(2,5-Dioxopyrrol-1-yl)ethoxy]ethoxy]acetyloxy-2,5-pyrrolidinedione; Mal-PEG2-OSu; Maleimide-PEG2-N-hydroxysuccinimide ester; SM(PEG)2
EINECS	Contact for details

Quality Control

Our Mal-PEG2-NHS Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the high standards required for sensitive bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.