Description

Azido-PEG5-NHS Ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring a terminal azide group and an N-hydroxysuccinimide (NHS) ester. This compound is essential for creating stable, site-specific conjugations between biomolecules via click chemistry and amine coupling. It is primarily needed by researchers and manufacturers in bioconjugation, pharmaceutical development, and advanced material science for constructing drug delivery systems, diagnostic probes, and functionalized surfaces.

Application

• Bioconjugation and ADC Development: Site-specific conjugation of antibodies, proteins, or peptides with payloads (e.g., drugs, toxins, dyes) via copper-free click chemistry with alkyne-modified molecules.
• PEGylation Linker: Introduction of a hydrophilic PEG spacer to improve the solubility, stability, and pharmacokinetic properties of therapeutic biomolecules.
• Surface Functionalization: Modification of nanoparticles, biosensors, or microarray surfaces with bioactive ligands for targeted binding and detection.
• Polymer Chemistry: Building block for synthesizing functionalized polymers and hydrogels with tailored biological activity.
• Diagnostic Probe Synthesis: Creation of imaging agents and contrast media by linking targeting moieties to reporter molecules.
• Chemical Biology Tools: Preparation of activity-based probes for studying enzyme function and protein-protein interactions in living systems.

Basic Information

Product Name	Azido-PEG5-NHS Ester
CAS No.	1433996-86-1
Molecular Formula	C22H38N4O10
Molecular Weight	518.56 g/mol
Synonyms	NHS-PEG5-Azide; Azido-PEG5-NHS Ester; 5-Azido-3,6,9,12,15-pentaoxaheptadecan-1-oic Acid 2,5-Dioxopyrrolidin-1-yl Ester; N-(Azido-PEG5)-N-hydroxysuccinimide Ester; α-Azido-ω-NHS-PEG5; PEG5-Azide-NHS; Azido-Pentaethylene Glycol-NHS Ester; N-Succinimidyl Azido-PEG5 Ester
EINECS	Contact for details

Quality Control

Our Azido-PEG5-NHS Ester is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional integrity. We adhere to cGMP principles where applicable to support pre-clinical and pharmaceutical development workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Assay (NMR)	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.