Description

Mal-Amido-Peg2-Tfp Ester is a heterobifunctional, amine-reactive crosslinking reagent featuring a maleimide group and a tetrafluorophenyl (TFP) ester, connected by a short, hydrophilic polyethylene glycol (PEG2) spacer. This compound is specifically engineered for the site-specific conjugation of biomolecules, enabling the creation of stable, well-defined bioconjugates critical for advanced research and therapeutic development. It is an essential tool for scientists and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic assay formulation, where precise and efficient coupling is paramount.

Application

• Antibody-Drug Conjugate (ADC) Linker Chemistry: Site-specific conjugation of cytotoxic payloads to antibodies via thiol groups, leveraging the maleimide-thiol click chemistry and the hydrolytically stable TFP ester-amine linkage.
• Protein and Peptide Labeling: Conjugation of fluorescent dyes, biotin, or other probes to cysteine-containing proteins for imaging, detection, and pull-down assays.
• Surface Functionalization: Immobilization of proteins or peptides onto amine-functionalized biosensor chips, nanoparticles, or solid supports to create bioactive surfaces.
• Polymer-Protein Hybrids: Synthesis of PEGylated proteins with controlled attachment points to improve pharmacokinetics and reduce immunogenicity.
• Diagnostic Reagent Development: Production of stable enzyme-antibody or hapten-carrier protein conjugates for ELISA and other immunoassays.
• Nanomedicine: Functionalization of liposomes or polymeric nanoparticles with targeting ligands (e.g., peptides, antibodies) for targeted drug delivery systems.

Basic Information

Item	Details
Product Name	Mal-Amido-Peg2-Tfp Ester
CAS No.	1431291-44-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Maleimide-PEG2-TFP Ester; TFP-PEG2-Maleimide; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethoxy]ethoxy]propanoate (TFP ester derivative); 1-(2-(2-(3-((2,3,5,6-Tetrafluorophenoxy)carbonyl)propoxy)ethoxy)ethyl)-1H-pyrrole-2,5-dione; Heterobifunctional PEG crosslinker, Maleimide and TFP ester; Amine and Thiol Reactive Crosslinker; SM(PEG)2 TFP Ester analog
EINECS	Contact for details

Quality Control

Our Mal-Amido-Peg2-Tfp Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment, NMR for structural confirmation, and mass spectrometry for identity verification. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all relevant specifications to ensure traceability and compliance with your research or cGMP guidelines for early-stage therapeutic development.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. Store away from strong bases and nucleophiles.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.