Description

Cyanine 5 Tyramide is a specialized, fluorescent tyramide reagent used in signal amplification for advanced molecular detection assays. Its core value lies in enabling highly sensitive, multiplexed detection of low-abundance targets in tissue and cell samples. This product is essential for researchers and diagnostic developers in the fields of histopathology, in situ hybridization (ISH), and immunohistochemistry (IHC) who require superior signal-to-noise ratios.

Application

• Tyramide Signal Amplification (TSA) for immunohistochemistry (IHC) and immunofluorescence (IF) staining.
• High-sensitivity multiplexed in situ hybridization (ISH) applications.
• Detection of low-copy-number RNA and DNA targets in formalin-fixed, paraffin-embedded (FFPE) tissue sections.
• Enhancing fluorescence signals in flow cytometry and microscopy for rare cell population analysis.
• Development of advanced molecular diagnostic assays and research kits.
• Use in combination with horseradish peroxidase (HRP) for catalyzed reporter deposition (CARD).

Basic Information

Product Name	Cyanine 5 Tyramide
CAS No.	1431148-26-3
Molecular Formula	C41H45ClN4O2
Molecular Weight	661.28 g/mol
Synonyms	Cy5 Tyramide; Tyramide-Cy5; Cyanine5 Tyramide; TSA-Cy5; HRP Tyramide-Cy5; N-(4-Hydroxyphenethyl)-2-((1E,3E)-5-((E)-1-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethyl-5-sulfo-3H-indol-1-ium-2-yl)penta-1,3-dien-1-yl)-3,3-dimethyl-1-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3H-indol-1-ium-5-sulfonate chloride; Tyramide conjugated to Cyanine 5
EINECS	Contact for details

Quality Control

Our Cyanine 5 Tyramide is manufactured under strict quality systems to ensure batch-to-batch consistency and performance in sensitive amplification assays. Each lot is characterized by HPLC, NMR, and mass spectrometry to confirm identity, purity, and labeling efficiency. Certificates of Analysis (COA) with detailed spectral data are provided to support your research and development compliance.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation. The material is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Dark blue to purple solid or film
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	646 - 652 nm
Emission Maximum	662 - 668 nm
Extinction Coefficient (ε)	≥ 250,000 L·mol⁻¹·cm⁻¹
Solubility	Soluble in DMSO, DMF
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.