Description

Propargyl-Peg3-Nhs Ester is a heterobifunctional crosslinker featuring a terminal alkyne (propargyl) group and an amine-reactive N-hydroxysuccinimide (NHS) ester. This compound is essential for advanced bioconjugation and click chemistry applications, enabling the precise, covalent attachment of biomolecules, polymers, and surfaces. It is a critical reagent for researchers and manufacturers in biopharmaceutical development, proteomics, and materials science who require reliable, high-purity building blocks for creating complex molecular architectures.

Application

• Bioconjugation and Antibody-Drug Conjugate (ADC) Development: Site-specific labeling of proteins, antibodies, and peptides via NHS-ester coupling to primary amines, followed by copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry.
• Polymer and Dendrimer Functionalization: Synthesis of functionalized polyethylene glycol (PEG) derivatives and smart polymers for drug delivery systems and surface modification.
• Surface Immobilization and Microarray Fabrication: Creating functionalized surfaces for biosensors, diagnostic assays, and cell culture substrates by linking amine-containing ligands.
• Chemical Biology and Proteomics: As a key reagent for activity-based protein profiling (ABPP) and the synthesis of chemical probes to study enzyme function and protein interactions.
• Nanoparticle and Liposome Modification: Conjugation of targeting ligands, imaging agents, or therapeutic payloads to nanocarrier platforms.
• Solid-Phase Peptide Synthesis (SPPS): Incorporation of alkyne handles into peptide sequences for subsequent on-resin or solution-phase conjugation.

Basic Information

Product Name	Propargyl-Peg3-Nhs Ester
CAS No.	1428629-71-3
Molecular Formula	C19H29NO8
Molecular Weight	399.44 g/mol
Synonyms	Propargyl-PEG3-NHS ester; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-(2-prop-2-ynoxyethoxy)ethoxy]ethoxy]propanoate; Propargyl-PEG3-NHS ester; NHS-PEG3-Propargyl; Alkyne-PEG3-NHS ester; 1-(2,5-Dioxopyrrolidin-1-yl)-3-[2-[2-(2-prop-2-yn-1-yloxyethoxy)ethoxy]ethoxy]propan-1-one; Propargyl-O-(CH2CH2O)3-CH2CH2CO2-NHS
EINECS	Contact for details

Quality Control

Our Propargyl-Peg3-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and batch-to-batch consistency suitable for sensitive research and development applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis of the NHS ester moiety.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous liquid or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.