Description

Cyanine5 Tetrazine CAS NO 1427705-31-4 is a high-performance, near-infrared fluorescent dye functionalized with a bioorthogonal tetrazine group. This compound matters for its critical role in advanced bioimaging and diagnostics, enabling rapid, catalyst-free click chemistry for labeling biomolecules in live cells. It is essential for researchers and developers in the pharmaceutical, biotechnology, and life science sectors who require precise, high-contrast imaging agents for drug discovery and diagnostic assay development.

Application

• Bioorthogonal Labeling: For the specific, rapid tagging of trans-cyclooctene (TCO)-modified proteins, antibodies, and oligonucleotides in live-cell imaging.
• In Vivo Imaging: Utilized in preclinical research for deep-tissue fluorescence imaging due to its near-infrared emission, minimizing background autofluorescence.
• Diagnostic Probe Development: Serves as a key component in the synthesis of targeted fluorescent probes for disease detection and biomarker analysis.
• Flow Cytometry & Microscopy: Used as a fluorescent conjugate for cell sorting (FACS) and high-resolution confocal or super-resolution microscopy applications.
• Chemical Biology Research: Enables the study of biomolecular interactions, trafficking, and localization through turn-on or turn-off fluorescence assays.
• Theragnostic Agent Synthesis: Incorporated into platforms that combine diagnostic imaging with therapeutic delivery.

Basic Information

Product Name	Cyanine5 Tetrazine
CAS No.	1427705-31-4
Molecular Formula	C41H45N7O22+ • 2X- (X = counterion)
Molecular Weight	Contact for details
Synonyms	Cy5 Tetrazine; Tetrazine-Cy5; Cy5-Tz; Sulfo-Cyanine5 Tetrazine (if sulfonated); 1-(6-(4-(1,2,4,5-Tetrazin-3-yl)benzylamino)-6-oxohexyl)-3,3-dimethyl-2-((E)-3-((E)-1,3,3-trimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium; Near-IR Tetrazine Dye; Bioorthogonal Cy5 Dye
EINECS	Contact for details

Quality Control

Our Cyanine5 Tetrazine is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and spectrophotometry to validate optical properties. Certificates of Analysis (COA) detailing all specifications are provided to ensure compliance with your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and degradation.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption Maximum (λmax)	646 - 652 nm
Emission Maximum (λem)	662 - 670 nm
Extinction Coefficient (ε)	≥ 250,000 M-1cm-1
Solubility	Soluble in DMSO, DMF, water (sulfonated form)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.