Description

Azide-PEG3-Desthiobiotin is a heterobifunctional crosslinker and labeling reagent that combines a click-reactive azide group with a high-affinity desthiobiotin tag via a triethylene glycol (PEG3) spacer. This compound matters for researchers and developers requiring efficient, reversible bioconjugation and affinity purification in complex biological systems. It is primarily needed by professionals in bioconjugation, proteomics, and drug discovery for labeling proteins, antibodies, and other biomolecules.

Application

• Bioconjugation and Protein Labeling: Site-specific modification of proteins, antibodies, and peptides via copper-free click chemistry with alkyne-functionalized targets.
• Affinity Purification and Pull-Down Assays: Reversible capture and isolation of labeled biomolecules using streptavidin/avidin resins, followed by gentle elution with biotin or desthiobiotin competitors.
• Proteomics Research: Enrichment of low-abundance proteins or post-translationally modified proteins for mass spectrometry analysis.
• Cell Surface Engineering: Labeling of cell surface glycans or proteins for imaging, tracking, or cell sorting applications.
• Diagnostic Assay Development: Conjugation of detection molecules (e.g., enzymes, fluorophores) to antibodies or other capture agents in immunoassays.
• ADC (Antibody-Drug Conjugate) Linker Exploration: Serves as a model or functional component in the development of cleavable linker systems for targeted drug delivery.
• Biomaterial Functionalization: Introduction of bioactive ligands onto nanoparticles, hydrogels, or other material surfaces for targeted interactions.

Basic Information

Product Name	Azide-PEG3-Desthiobiotin
CAS No.	1426828-06-9
Molecular Formula	C₂₀H₃₇N₇O₅S
Molecular Weight	487.62 g/mol
Synonyms	N-(3-Azidopropyl)-23-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-5,8,11,14,17-pentaoxo-3,6,9,12,15-pentaoxa-2-azaoctadecan-18-amide; Desthiobiotin-PEG3-azide; 1-[2-[2-[2-[2-[5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]ethoxy]ethyl]-3-azidopropyl-urea; Azido-PEG3-Desthiobiotin; N-(Azido-PEG3)-N'-Desthiobiotin urea; Azide-PEG3-DTB; Click Chemistry Desthiobiotin Reagent
EINECS	Contact for details

Quality Control

Our Azide-PEG3-Desthiobiotin is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance. Certificates of Analysis (COA) with detailed specifications are available upon request to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. Allow the product to equilibrate to room temperature before opening to minimize moisture absorption. Store away from strong oxidizing agents.

Specification

Item	Specification
Appearance	White to off-white solid or powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; partially soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.