Description

Sulfo-Cyanine3 NHS Ester is a water-soluble, amine-reactive fluorescent labeling reagent designed for the preparation of stable bioconjugates. This compound is essential for researchers and developers requiring high-sensitivity detection and imaging in aqueous biological systems without compromising solubility. It is primarily needed by professionals in biotechnology, pharmaceutical R&D, and diagnostic kit manufacturing for tagging proteins, antibodies, peptides, and other biomolecules.

Application

• Antibody and Protein Labeling: For fluorescence-activated cell sorting (FACS), immunofluorescence (IF), and Western blotting applications.
• Nucleic Acid Probes: Conjugation to oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR detection.
• Live-Cell Imaging: Preparation of fluorescent ligands and substrates for tracking biological processes in live cells.
• Diagnostic Assay Development: Manufacturing fluorescent conjugates for ELISA, lateral flow assays, and other clinical diagnostics.
• Biomolecular Interaction Studies: Labeling proteins for fluorescence polarization (FP) and Förster resonance energy transfer (FRET) assays.
• Proteomics and Biomarker Research: Tagging for gel-based and microarray-based proteomic analysis.

Basic Information

Product Name	Sulfo-Cyanine3 NHS Ester
CAS No.	1424433-17-9
Molecular Formula	C37H41KN2O10S2
Molecular Weight	777.0 g/mol
Synonyms	Sulfo-Cy3 NHS Ester; Cyanine3 sulfonyl NHS ester; Sulfo-Cy3 SE; 2,5-Dioxopyrrolidin-1-yl 1-(6-((E)-2-((E)-3-(2-((E)-1-(5-carboxypentyl)-3,3-dimethyl-5-sulfoindolin-2-ylidene)ethylidene)-2-((E)-1-(5-carboxypentyl)-3,3-dimethylindolin-2-ylidene)cyclopent-1-en-1-yl)vinyl)-1-ethyl-3,3-dimethyl-3H-indol-1-ium-5-yl)butane-1-sulfonate; 1-(5-Carboxypentyl)-2-((E)-3-((E)-2-((1E,3E)-3-(1-ethyl-5-sulfonatobutyl-3,3-dimethylindolin-2-ylidene)prop-1-en-1-yl)cyclopent-1-en-1-yl)vinyl)-3,3-dimethyl-3H-indol-1-ium-5-sulfonate NHS ester
EINECS	Contact for details

Quality Control

Our Sulfo-Cyanine3 NHS Ester is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure consistent high performance. Certificates of Analysis (COA) with detailed specifications are provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Extinction Coefficient (ε)	~150,000 cm-1M-1 at 554 nm
Fluorescence Emission	~570 nm
Solubility	Soluble in water, DMSO, DMF
Single Spot (TLC)	Conforms
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.