Description

Sulfo Cyanine3 NHS Ester is a water-soluble, amine-reactive fluorescent labeling reagent widely used in bioconjugation. Its core value lies in providing a bright, stable, and pH-insensitive near-infrared fluorescence signal, enabling highly sensitive detection in complex biological matrices. This product is essential for researchers and manufacturers in the fields of biopharmaceutical development, in vitro diagnostics (IVD), and life science research, where reliable protein and antibody labeling is critical.

Application

• Antibody & Protein Labeling: Conjugation to monoclonal antibodies, proteins, and peptides for fluorescence-based assays and imaging.
• Biopharmaceutical Analysis: Development and quality control of antibody-drug conjugates (ADCs) and other biologics.
• In Vitro Diagnostics (IVD): Manufacturing of fluorescent probes and reagents for immunoassays, flow cytometry, and lateral flow tests.
• Biomolecular Imaging: Labeling of biomolecules for fluorescence microscopy (e.g., confocal, super-resolution) and in vivo imaging applications.
• Nucleic Acid Detection: Preparation of labeled oligonucleotide probes for fluorescence in situ hybridization (FISH) and PCR.
• Cellular & Tissue Staining: Conjugation to targeting ligands for specific cell surface or intracellular staining.
• High-Throughput Screening (HTS): Development of fluorescent tracers and sensors for drug discovery platforms.
• Material Science: Functionalization of nanoparticles and polymers for bioimaging and theranostic applications.

Basic Information

Product Name	Sulfo Cyanine3 NHS Ester
CAS No.	1424150-38-8
Molecular Formula	C37H41KN2O10S2
Molecular Weight	777.0 g/mol
Synonyms	Sulfo-Cy3 NHS Ester; Cyanine3 sulfonyl NHS ester; Sulfo Cy3 NHS ester; 2,5-Dioxopyrrolidin-1-yl 1-(6-((E)-2-((E)-3,3-dimethyl-5-sulfo-1-(4-sulfobutyl)indolin-2-ylidene)ethylidene)-2-((E)-1-(2,5-dioxopyrrolidin-1-yl)-3-oxo-6,9,12,15-tetraoxa-2-azaoctadecan-18-amido)-3,3-dimethyl-3H-indol-1-ium-5-yl)hexane-1,6-diyl (2S)-2-(6-((E)-2-((E)-3,3-dimethyl-5-sulfo-1-(4-sulfobutyl)indolin-2-ylidene)ethylidene)-2-((E)-1-(2,5-dioxopyrrolidin-1-yl)-3-oxo-6,9,12,15-tetraoxa-2-azaoctadecan-18-amido)-3,3-dimethyl-3H-indol-1-ium-5-yl)acetate (Potassium Salt); Sulfo-Cyanine3 N-hydroxysuccinimide ester; Water-soluble Cy3 NHS ester
EINECS	Contact for details

Quality Control

Our Sulfo Cyanine3 NHS Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and spectrophotometry for labeling efficiency verification. Certificates of Analysis (COA) detailing purity, absorbance, fluorescence, and moisture content are provided with every shipment to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, desiccate at low temperature. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark green to purple solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	548 - 552 nm
Emission Maximum	562 - 568 nm
Extinction Coefficient (ε)	≥ 150,000 cm⁻¹M⁻¹
Fluorescence Quantum Yield	Contact for details
Water Content (KF)	≤ 5.0%
Single Spot (TLC)	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.