Description

Azido-PEG4-amido-tri-(t-butoxycarbonylethoxymethyl)-methane is a multifunctional, heterobifunctional PEG-based crosslinker and building block designed for advanced bioconjugation and materials science applications. This compound is engineered to provide a precise, multi-armed architecture, enabling the controlled attachment of biomolecules or other functional groups through its azide and protected carboxylate termini. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require sophisticated linkers for drug delivery systems, surface functionalization, and the synthesis of complex biomaterials.

Application

• Bioconjugation and ADC Development: Serves as a key linker for attaching cytotoxic drugs to antibodies (Antibody-Drug Conjugates) or other targeting moieties via click chemistry (azide-alkyne cycloaddition).
• Polymer and Dendrimer Synthesis: Acts as a core building block for creating multi-armed, PEGylated polymers and dendrimers with protected functional groups for sequential modification.
• Surface Modification and Immobilization: Used to functionalize nanoparticles, biosensor chips, and other solid supports with azide groups for subsequent bio-orthogonal coupling.
• Peptide and Protein Modification: Enables site-specific PEGylation or the introduction of multiple protected carboxyl groups onto peptides and proteins to alter solubility, stability, and pharmacokinetics.
• Materials Science and Hydrogel Formation: Employed as a crosslinking agent in the development of biocompatible hydrogels and smart materials for tissue engineering and controlled release.
• Diagnostic Reagent Preparation: Facilitates the creation of labeled probes and assay components through its reactive handles for fluorescence or other detection methods.

Basic Information

Product Name	Azido-PEG4-amido-tri-(t-butoxycarbonylethoxymethyl)-methane
CAS No.	1421933-29-0
Molecular Formula	C40H73N5O16
Molecular Weight	880.03 g/mol
Synonyms	Azido-PEG4-amine tris-Boc acid; 2,2',2''-((((1-Azido-3,6,9,12-tetraoxapentadecan-15-yl)amino)methylidene)tris(ethane-2,1-diyl)) tris(2-methylpropane-2-carboxylate); Azido-PEG4-C3-acid tris(t-butyl ester); N1-(Azido-PEG4)-N1-{tris[2-(tert-butoxycarbonyl)ethyl]methyl}amine; Heterotrifunctional PEG linker, Azide/Tris-Boc; 15-Azido-4,7,10,13-tetraoxa-1-azahexadecan-1-yl-tris(2-((tert-butoxy)carbonyl)ethyl)methane
EINECS	Contact for details

Quality Control

Our Azido-PEG4-amido-tri-(t-butoxycarbonylethoxymethyl)-methane is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment, NMR (1H, 13C) for structural confirmation, and mass spectrometry for identity verification. We provide comprehensive Certificates of Analysis (COA) with detailed specifications upon request, ensuring traceability and compliance with research and cGMP-grade material standards where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) for long-term stability. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. Keep the container in a dry, well-ventilated area.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous liquid or oil
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.