Description

Endo-Bcn-Peg2-Pfp Ester CAS NO 1421932-53-7 is a high-purity, heterobifunctional polyethylene glycol (PEG) crosslinker featuring an endo-BCN group and a pentafluorophenyl (PFP) ester terminus. This compound is specifically engineered for efficient, copper-free click chemistry bioconjugation, enabling the stable and rapid attachment of biomolecules under mild, aqueous conditions. It is an essential reagent for researchers and manufacturers in pharmaceutical development, proteomics, and advanced material science, where precise and biocompatible ligation strategies are required.

Application

• Antibody-Drug Conjugate (ADC) Development: For site-specific conjugation of cytotoxic payloads to monoclonal antibodies via strain-promoted azide-alkyne cycloaddition (SPAAC).
• PEGylation of Peptides and Proteins: To introduce polyethylene glycol chains via the reactive PFP ester, improving pharmacokinetic properties such as solubility and circulation half-life.
• Surface Functionalization: For modifying nanoparticles, biosensors, and microarray slides with bioorthogonal handles for subsequent capture or labeling.
• Polymer and Hydrogel Synthesis: As a crosslinking agent in the creation of smart biomaterials and drug delivery scaffolds.
• Diagnostic Reagent Preparation: In the synthesis of fluorescent or enzyme-labeled probes for imaging and detection assays.
• Chemical Biology Tool Development: For creating activity-based probes or affinity tags to study protein function and interactions in live cells.

Basic Information

Product Name	Endo-Bcn-Peg2-Pfp Ester
CAS No.	1421932-53-7
Molecular Formula	C₂₄H₂₉F₅N₂O₆
Molecular Weight	536.50 g/mol
Synonyms	endo-BCN-PEG2-PFP ester; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (1-oxo-2,5,8,11-tetraoxatetradecan-14-yl) (2,3,4,5,6-pentafluorophenyl) carbonate; BCN-PEG2-PFP; BCN-PEG2-PFP ester; 2,5,8,11-Tetraoxatetradecan-14-oic acid, 14-[[(2,3,4,5,6-pentafluorophenoxy)carbonyl]oxy]-, (1R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-ylmethyl ester; BCN-PEG2-PFP Crosslinker
EINECS	Contact for details

Quality Control

Our Endo-Bcn-Peg2-Pfp Ester is manufactured under strict quality management systems. Every batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure identity, purity, and consistency. Certificates of Analysis (COA) detailing all specifications are provided with each shipment and are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.