Description

Cy5 Acid(Tri So3) is a highly functionalized, water-soluble cyanine dye derivative bearing three sulfonate groups. This chemical structure is critical for enhancing solubility in aqueous buffers and reducing non-specific binding, which is essential for sensitive detection applications. It is primarily used by researchers and manufacturers in the life sciences, diagnostics, and bioconjugation sectors who require stable, bright fluorescent labeling for proteins, antibodies, and oligonucleotides.

Application

• Bioconjugation & Labeling: Covalent attachment to biomolecules (e.g., antibodies, proteins, peptides) for fluorescence-activated cell sorting (FACS), immunohistochemistry (IHC), and in situ hybridization (ISH).
• Diagnostic Assay Development: Used as a critical fluorescent probe in the development of ELISA, lateral flow assays, and other clinical diagnostic kits.
• Nucleic Acid Labeling: Labeling of oligonucleotides for fluorescence in situ hybridization (FISH) probes and real-time PCR applications.
• In Vivo Imaging: Conjugation for near-infrared (NIR) fluorescence imaging in preclinical research due to its favorable excitation/emission profiles.
• Material Science: Incorporation into polymers and nanoparticles to create functional, traceable materials for drug delivery research.

Basic Information

Product Name	Cy5 Acid(Tri So3)
CAS No.	1416048-57-1
Molecular Formula	C40H45N3O13S33- • 3X+ (X = counter ion)
Molecular Weight	Contact for details
Synonyms	Sulfo-Cyanine5 acid; Cy5™ trisulfonate; 1H-Benz[e]indolium, 2-[5-[1,1-dimethyl-3-(4-sulfobutyl)-1,3-dihydro-2H-benz[e]indol-2-ylidene]-1,3-pentadien-1-yl]-1,1-dimethyl-3-(4-sulfobutyl)-, inner salt, sulfate (1:?); Cy5 carboxylic acid trisulfonate; Trisulfonated Cy5 NHS ester precursor; Water-soluble Cy5 acid; Near-IR fluorescent dye acid
EINECS	Contact for details

Quality Control

Our Cy5 Acid(Tri So3) is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing to ensure high purity, consistent performance, and batch-to-batch reproducibility critical for research and diagnostic applications. Certificates of Analysis (COA) with detailed HPLC, NMR, and MS data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. For short-term use, the product may be stored at 2-8°C. Allow the vial to warm to room temperature in a dry environment before opening to prevent condensation.

Specification

Item	Specification
Appearance	Dark blue to purple solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Absorption (λmax)	646 - 652 nm
Emission (λmax)	662 - 668 nm
Extinction Coefficient (ε)	≥ 250,000 L·mol⁻¹·cm⁻¹

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.