Description

Fmoc-Peg5-Nhs Ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and an acid-labile Fmoc-protected amine. This compound is essential for creating stable, hydrophilic, and biocompatible spacers in bioconjugation and peptide synthesis, enabling precise control over molecular architecture. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and advanced materials sectors for developing drug delivery systems, diagnostic reagents, and functional biomaterials. Its design facilitates the controlled attachment of bioactive molecules while maintaining solubility and reducing immunogenicity.

Application

• Bioconjugation and Linker Chemistry: Used to covalently attach peptides, proteins, or other amine-containing molecules to surfaces, nanoparticles, or other biomolecules via its NHS ester group, with the Fmoc group providing a protected handle for further orthogonal chemistry.
• Peptide Synthesis (SPPS): Serves as a building block for introducing hydrophilic PEG spacers into synthetic peptides during solid-phase synthesis, improving peptide solubility and bioavailability.
• Drug Delivery System Development: Employed in the synthesis of prodrugs and antibody-drug conjugates (ADCs) to create cleavable, hydrophilic linkers between the drug payload and the targeting moiety.
• Diagnostic Reagent Fabrication: Facilitates the labeling of antibodies, enzymes, or oligonucleotides with fluorophores, haptens, or other reporter molecules for ELISA, flow cytometry, and in-vivo imaging applications.
• Hydrogel and Biomaterial Functionalization: Used to modify polymer backbones or material surfaces with bioactive peptides or growth factors to create tailored cell culture scaffolds and tissue engineering matrices.
• Surface Plasmon Resonance (SPR) and Biosensor Chip Coating: Applied to immobilize ligands on sensor surfaces via amine coupling, with the PEG spacer reducing non-specific binding.

Basic Information

Product Name	Fmoc-Peg5-Nhs Ester
CAS No.	1402080-11-8
Molecular Formula	C₃₄H₄₄N₂O₁₂
Molecular Weight	672.73 g/mol
Synonyms	Fmoc-NH-PEG5-NHS Ester; Fmoc-Amino-PEG5-NHS Ester; N-(9-Fluorenylmethoxycarbonyl)-3,6,9,12,15-pentaoxa-2-azaheptadecan-17-oic Acid Succinimidyl Ester; 2,5-Dioxopyrrolidin-1-yl 17-(((9H-fluoren-9-yl)methoxy)carbonyl)-3,6,9,12,15-pentaoxa-2-azaheptadecan-1-oate; Fmoc-PEG5-OSu; Fmoc-15-amino-4,7,10,13-tetraoxapentadecanoic Acid NHS Ester; Fmoc-N-amido-PEG5-NHS
EINECS	Contact for details

Quality Control

Our Fmoc-Peg5-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and bioconjugation applications. Certificates of Analysis (COA) detailing purity, water content, and other critical parameters are provided with every shipment and are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize condensation and hydrolysis. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.