Description

Azido-Peg4-Amido-Tri-(Carboxyethoxymethyl)-Methane is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide group and a tri-branched carboxylic acid terminus. This compound matters for its unique ability to facilitate click chemistry via its azide functionality while providing multiple sites for conjugation through its carboxyl groups, enabling the creation of complex, multi-armed molecular architectures. It is primarily needed by researchers and manufacturers in bioconjugation, pharmaceutical development, and advanced materials science for constructing drug delivery systems, diagnostic probes, and hydrogel networks.

Application

• Bioconjugation and Linker Chemistry: Used to attach biomolecules (peptides, proteins, antibodies) to surfaces, nanoparticles, or other polymers via stable amide or ester bonds.
• Drug Delivery System Development: Serves as a key building block for synthesizing multi-arm PEG-based drug carriers, enhancing solubility and pharmacokinetics of active pharmaceutical ingredients (APIs).
• Click Chemistry Scaffolding: The azide group enables copper-catalyzed or strain-promoted azide-alkyne cycloaddition (CuAAC/SPAAC) for modular assembly of complex molecules.
• Hydrogel Formation: The multiple carboxyl groups allow for crosslinking with amine-containing polymers to form biocompatible hydrogels for tissue engineering and 3D cell culture.
• Diagnostic Reagent Synthesis: Employed in the preparation of contrast agents, fluorescent probes, and other diagnostic tools requiring precise, multi-point attachment.
• Surface Functionalization: Used to modify material surfaces (e.g., gold, silica) with a dense, functional PEG layer to create non-fouling or specifically reactive interfaces.

Basic Information

Product Name	Azido-Peg4-Amido-Tri-(Carboxyethoxymethyl)-Methane
CAS No.	1398044-51-3
Molecular Formula	C26H45N5O13
Molecular Weight	635.66 g/mol
Synonyms	Azido-PEG4-amine-Tris(acetic acid); N3-PEG4-Tris-NTA; Azido-PEG4-tris-NTA; 2,2',2''-Nitrilotriacetic acid, N-[2-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethyl]-, trisodium salt; Azido-PEG4-amido-tris(carboxymethyl)amine; Tris-NTA-PEG4-azide; Heterotrifunctional PEG crosslinker, Azide/COOH; 1-Azido-3,6,9,12-tetraoxapentadecan-15-amido-tri(carboxymethyl)methane
EINECS	Contact for details

Quality Control

Our Azido-Peg4-Amido-Tri-(Carboxyethoxymethyl)-Methane is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to confirm identity, purity, and functional group integrity. Certificates of Analysis (COA) with detailed chromatographic data are provided to ensure compliance with your research and development specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous liquid or oil
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Assay (Azide)	≥ 90% of theoretical

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.