Description

Cyanine3 NHS Ester is a reactive, amine-reactive fluorescent labeling reagent widely used in life science research. Its core value lies in providing a bright, photostable red-fluorescent tag for biomolecules, enabling sensitive detection and imaging. This product is essential for researchers and manufacturers in bioconjugation, flow cytometry, fluorescence microscopy, and in vitro diagnostics who require reliable and high-performance labeling tools.

Application

• Antibody and Protein Labeling: Conjugation to primary amines (lysine residues) for creating fluorescent probes used in immunofluorescence, Western blotting, and ELISA.
• Nucleic Acid Detection: Labeling of amine-modified oligonucleotides for fluorescence in situ hybridization (FISH) and microarray applications.
• Cell and Tissue Imaging: Preparation of labeled peptides, streptavidin, or other targeting molecules for high-contrast cellular and subcellular imaging.
• Flow Cytometry: Development of fluorescent antibody conjugates and other cell surface markers for multiparameter analysis.
• Biomaterial and Nanoparticle Functionalization: Covalent attachment of fluorescent tags to polymers, dendrimers, or nanoparticle surfaces for tracking and biodistribution studies.
• Proteomics and Biomarker Research: Fluorescent labeling of proteins for gel-based analysis, activity assays, and protein interaction studies.

Basic Information

Product Name	Cyanine3 NHS Ester
CAS No.	1393363-07-9
Molecular Formula	C37H41N3O6
Molecular Weight	623.75 g/mol
Synonyms	Cy3 NHS Ester; Cy3 N-Hydroxysuccinimide Ester; Cy3 Succinimidyl Ester; 1-(5-Carboxypentyl)-1'-ethyl-3,3,3',3'-tetramethylindocarbocyanine N-hydroxysuccinimide ester; Sulfo-Cy3 NHS Ester (note: sulfonated variant); Cyanine 3 carboxylic acid N-succinimidyl ester; Cy3® NHS ester (trade name); 2-[(1E,3E)-5-[(1E,3E)-3-(1-Ethyl-3,3-dimethyl-5-sulfoindolin-2-ylidene)prop-1-en-1-yl]-3-(1-ethyl-3,3-dimethylindolin-2-ylidene)penta-1,3-dien-1-yl]-1-ethyl-3,3-dimethyl-3H-indol-1-ium-5-sulfonate NHS ester
EINECS	Contact for details

Quality Control

Our Cyanine3 NHS Ester is produced under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent performance, and batch-to-batch reproducibility. Certificates of Analysis (COA) detailing purity, absorbance, fluorescence, and functional performance are available upon request. We support research and diagnostic applications requiring reliable and traceable reagents.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	Dark red to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	548 - 552 nm
Emission Maximum	562 - 568 nm
Extinction Coefficient (ε)	≥ 150,000 cm⁻¹M⁻¹
Solubility	Soluble in DMF, DMSO, acetonitrile
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.