Description

Tetrazine Active Ester (Tetrazine-NHS) is a high-value, heterobifunctional crosslinking reagent featuring a highly reactive s-tetrazine moiety and an N-hydroxysuccinimide (NHS) ester group. This compound is essential for bioorthogonal chemistry, enabling rapid, catalyst-free, and selective conjugation reactions under mild physiological conditions. It is primarily utilized by researchers and manufacturers in the pharmaceutical, bioconjugation, and advanced materials sectors for labeling proteins, antibodies, and nanoparticles, as well as for developing targeted therapeutics and diagnostic probes.

Application

• Bioorthogonal Labeling and Imaging: Conjugation of tetrazine probes to biomolecules for real-time, high-contrast imaging in live cells and tissues via inverse electron-demand Diels-Alder (IEDDA) reactions.
• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies to create next-generation, homogeneous ADCs with improved therapeutic indices.
• Protein and Peptide Modification: Efficient labeling of proteins, enzymes, and peptides with fluorophores, affinity tags, or other functional molecules for proteomics and structural biology studies.
• Surface Functionalization: Modification of nanoparticles, polymers, and solid supports for creating bioactive surfaces used in biosensors, diagnostics, and tissue engineering.
• Pre-targeting Strategies in Radioimmunotherapy: Key component in multi-step pre-targeting approaches to improve tumor targeting and reduce off-target radiation exposure.
• Chemical Biology Tool Development: Synthesis of novel chemical probes for studying biological processes, protein-protein interactions, and enzyme activity.

Basic Information

Product Name	Tetrazine Active Ester (Tetrazine-NHS)
CAS No.	1384271-41-3
Molecular Formula	C16H16N6O4
Molecular Weight	356.34 g/mol
Synonyms	3-(4-(6-Methyl-1,2,4,5-tetrazin-3-yl)phenyl)propanoic acid N-hydroxysuccinimide ester; Tetrazine-NHS ester; 6-Methyl-3-(4-(3-((2,5-dioxopyrrolidin-1-yl)oxy)-3-oxopropyl)phenyl)-1,2,4,5-tetrazine; s-Tetrazine-NHS; Methyltetrazine-NHS Ester; TZ-NHS; Bioorthogonal Tetrazine Crosslinker
EINECS	Contact for details

Quality Control

Our Tetrazine Active Ester is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high purity, critical for sensitive bioorthogonal applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to cGMP principles where applicable, and our quality system is designed to meet the stringent requirements of research and pre-clinical development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	Red to purple solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.