Description

Dbco-Peg5-Nhs Ester is a heterobifunctional crosslinker featuring a dibenzocyclooctyne (DBCO) group for strain-promoted azide-alkyne cycloaddition (SPAAC) and an N-hydroxysuccinimide (NHS) ester for amine coupling. This compound is essential for creating stable, bioconjugate linkages without the need for cytotoxic copper catalysts, enabling efficient and selective labeling of biomolecules. It is a critical reagent for researchers and developers in bioconjugation, antibody-drug conjugate (ADC) development, and the synthesis of peptide and protein therapeutics.

Application

• Antibody-Drug Conjugate (ADC) Synthesis: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies via amine groups.
• Fluorescent Labeling & Imaging Probes: Conjugation of dyes and imaging agents to proteins, peptides, or nanoparticles for in vitro and in vivo diagnostics.
• PEGylation & Biopolymer Modification: Introduction of polyethylene glycol (PEG) chains or other functional groups to enhance the stability and pharmacokinetics of therapeutic proteins.
• Surface Functionalization: Immobilization of biomolecules onto solid supports (e.g., biosensors, microarray slides, magnetic beads) for assay development.
• Click Chemistry for Material Science: Building block for creating functionalized hydrogels, polymers, and nanomaterials through bioorthogonal SPAAC reactions.
• Small Molecule-Protein Conjugates: Creation of hapten-carrier protein complexes for vaccine and immunoassay development.

Basic Information

Product Name	Dbco-Peg5-Nhs Ester
CAS No.	1378531-80-6
Molecular Formula	C37H45N3O11
Molecular Weight	707.77 g/mol
Synonyms	DBCO-PEG5-NHS Ester; DBCO-PEG5-NHS Ester; Dibenzocyclooctyne-PEG5-N-hydroxysuccinimide Ester; DBCO-PEG5-NHS; 1-(11,12-Didehydrodibenzo[b,f]azocin-5(6H)-yl)-3,6,9,12,15-pentaoxa-2-azaheptadecan-17-oic Acid 2,5-Dioxopyrrolidin-1-yl Ester; DBCO-Amine PEG NHS Ester; DBCO-NHS Ester PEG5; Azadibenzocyclooctyne-PEG5-NHS
EINECS	Contact for details

Quality Control

Our Dbco-Peg5-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance for sensitive bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional integrity is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Activity	Conjugation efficiency confirmed by model reaction

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.