Description

AF488 NHS Ester is a high-purity, amine-reactive fluorescent labeling reagent widely used in life science research and diagnostics. This compound is critical for creating stable, bright conjugates with proteins, antibodies, and other biomolecules for sensitive detection and imaging applications. It is an essential tool for researchers and manufacturers in biotechnology, pharmaceutical development, and diagnostic kit production who require reliable and efficient fluorescent probes.

Application

• Antibody and Protein Labeling: Conjugation to primary or secondary antibodies for fluorescence microscopy (FLM), flow cytometry, and immunofluorescence (IF) assays.
• Biomolecule Tagging for Bioassays: Labeling of peptides, oligonucleotides, or other probes for use in fluorescence polarization (FP), Förster resonance energy transfer (FRET), and high-throughput screening (HTS) applications.
• Diagnostic Reagent Manufacturing: Production of fluorescent conjugates for in vitro diagnostic (IVD) devices, lateral flow assays, and ELISA kits.
• Cellular Imaging and Tracking: Labeling of cellular components or entire cells for real-time visualization and tracking in live-cell imaging studies.
• Bioconjugation and Probe Development: A key building block for creating custom fluorescent sensors, molecular beacons, and targeted imaging agents.
• Western Blotting: Preparation of fluorescently-labeled protein markers or detection reagents for sensitive gel-based analysis.

Basic Information

Item	Detail
Product Name	AF488 NHS Ester
CAS No.	1374019-99-4
Molecular Formula	C31H28N4O8
Molecular Weight	584.58 g/mol
Synonyms	Alexa Fluor® 488 NHS Ester (Succinimidyl Ester); Alexa Fluor 488 SE; 6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxo-2-((3S,5R,6S)-5-((3-oxo-3H-benzo[d]xanthen-6-yl)amino)-6-((4-sulfophenyl)amino)-3H-xanthen-3-yl)hexanoic acid; AF488 NHS; AF488 Succinimidyl Ester; Alexa Fluor® 488 carboxylic acid succinimidyl ester
EINECS	Contact for details

Quality Control

Every batch of AF488 NHS Ester is manufactured and tested under strict quality management protocols to ensure high purity, consistent performance, and batch-to-batch reproducibility. Our quality control includes rigorous analytical testing by HPLC and spectroscopic methods. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance, fluorescence, and functional performance is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 90%
Absorption Maximum (λmax)	495 ± 3 nm
Emission Maximum	519 ± 3 nm
Extinction Coefficient (ε)	≥ 70,000 cm⁻¹M⁻¹
Fluorescence Quantum Yield (φ)	≥ 0.8
Solubility	Soluble in DMSO, DMF
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.