Description

Cyanine 5 Monofunctional Hexanoic Acid Dye, Succinimidyl Ester, Potassium Salt is a near-infrared fluorescent labeling reagent designed for the covalent conjugation of biomolecules. Its core value lies in providing a stable, water-soluble, and highly fluorescent tag for sensitive detection and imaging applications. This product is essential for researchers and manufacturers in the fields of bioconjugation, flow cytometry, in vivo imaging, and diagnostic assay development who require reliable and consistent fluorescent probes.

Application

• Bioconjugation and Labeling: Covalent attachment to proteins, antibodies, peptides, and oligonucleotides via primary amines (lysine residues or N-termini).
• Flow Cytometry & Cell Analysis: Fluorescent tagging of cell surface markers or intracellular targets for high-sensitivity detection.
• In Vivo Imaging: Conjugation to targeting agents (e.g., antibodies, peptides) for near-infrared fluorescence (NIRF) imaging in preclinical research.
• Diagnostic Assay Development: Used as a detection label in immunoassays (ELISA, lateral flow) and nucleic acid hybridization assays.
• Microscopy & Bioimaging: Fluorescent staining for fixed-cell imaging and super-resolution microscopy techniques.
• Drug Delivery & Pharmacokinetics: Tracking and visualization of conjugated therapeutic agents in biological systems.

Basic Information

Product Name	Cyanine 5 Monofunctional Hexanoic Acid Dye, Succinimidyl Ester, Potassium Salt
CAS No.	1358906-77-0
Molecular Formula	C38H45KN4O6
Molecular Weight	692.90 g/mol
Synonyms	Cy5 NHS Ester, Potassium Salt; Cy5™ Mono NHS Ester; Sulfo-Cyanine5 NHS ester; Cyanine5 carboxylic acid, succinimidyl ester, potassium salt; 1-(5-Carboxypentyl)-2-((1E,3E)-5-((E)-1,3-dihydro-3,3-dimethyl-1-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-2H-indol-2-ylidene)penta-1,3-dien-1-yl)-3,3-dimethyl-3H-indol-1-ium, potassium salt; N-Hydroxysuccinimide ester of Cy5; Cy5 SE
EINECS	Contact for details

Quality Control

Our Cyanine 5 NHS Ester is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure consistent performance and lot-to-lot reproducibility. Certificates of Analysis (COA) detailing purity, absorbance, fluorescence, and other critical parameters are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark blue to purple solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	646 - 652 nm
Emission Maximum (λem)	662 - 668 nm
Extinction Coefficient (ε)	≥ 250,000 L·mol⁻¹·cm⁻¹
Water Content (KF)	≤ 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.