Description

Fmoc-Cit-Opfp CAS NO 1356537-03-5 is a specialized, protected amino acid derivative used as a critical building block in solid-phase peptide synthesis (SPPS). Its value lies in enabling the efficient and selective incorporation of the amino acid citrulline into complex peptide sequences, which is essential for research in immunology and drug discovery. This high-purity reagent is primarily needed by pharmaceutical R&D laboratories, biotechnology companies, and academic institutions developing peptide-based therapeutics, diagnostics, and biochemical tools.

Application

• Solid-Phase Peptide Synthesis (SPPS): A key reagent for the controlled, sequential assembly of peptides, particularly for introducing citrulline residues.
• Peptide Drug Discovery & Development: Used in the research and production of peptide-based pharmaceuticals targeting autoimmune diseases, cancer, and inflammatory conditions.
• Citrullinated Peptide Antigen Production: Essential for synthesizing antigens used in research and diagnostic assays for rheumatoid arthritis and other autoimmune disorders.
• Biochemical Research: Employed in studying protein citrullination (deimination), a crucial post-translational modification in epigenetics and cell signaling.
• Custom Peptide Service Providers: A fundamental raw material for CROs and CDMOs offering bespoke peptide synthesis services to the life sciences industry.
• Protecting Group Chemistry: Serves as an example of orthogonal protection strategies using Fmoc (9-fluorenylmethoxycarbonyl) and Opfp (pentafluorophenyl ester) groups for carboxylic acid activation.

Basic Information

Product Name	Fmoc-Cit-Opfp
CAS No.	1356537-03-5
Molecular Formula	C₂₆H₂₀F₅NO₆
Molecular Weight	537.44 g/mol
Synonyms	Fmoc-Citrulline-OPfp; N-α-Fmoc-N-δ-(tert-Butoxycarbonyl)-L-citrulline pentafluorophenyl ester; Fmoc-Cit(Boc)-OPfp; (S)-2-(((9H-Fluoren-9-yl)methoxy)carbonyl)amino)-5-((tert-butoxycarbonyl)amino)pentanoic acid 2,3,4,5,6-pentafluorophenyl ester; Fmoc-L-Citrulline(Boc)-OPfp ester; N-α-Fmoc-N-ω-Boc-L-citrulline pentafluorophenyl ester; Boc-Cit(Fmoc)-OPfp (common alternate ordering); Pentafluorophenyl N-[(9H-fluoren-9-ylmethoxy)carbonyl]-N'-[(tert-butoxycarbonyl]-L-citrullinate
EINECS	Contact for details

Quality Control

Our Fmoc-Cit-Opfp is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, critical for sensitive peptide synthesis applications. Each lot undergoes comprehensive analytical testing including HPLC for purity, chiral analysis, and identity confirmation. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting compliance with our stringent specifications. We support cGMP manufacturing for advanced pharmaceutical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture ingress. For long-term stability, maintain storage conditions consistently.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (IR)	Spectrum matches reference
Purity (HPLC)	≥ 98.0%
Chiral Purity (HPLC)	≥ 99.0% (L-isomer)
Water Content (KF)	≤ 0.5%
Heavy Metals	≤ 10 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.