Description

Dbco-Nhs Ester is a high-purity, bifunctional chemical linker that combines a dibenzocyclooctyne (DBCO) group with an N-hydroxysuccinimide (NHS) ester. This compound is essential for enabling efficient, copper-free click chemistry bioconjugation reactions under mild, physiological conditions. It is primarily used by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostics industries for labeling proteins, antibodies, peptides, and other biomolecules without the need for cytotoxic metal catalysts.

Application

• Bioconjugation and Protein Labeling: Covalently attaching fluorescent dyes, drugs, or other functional molecules to antibodies, proteins, and peptides for therapeutic or diagnostic applications.
• ADC (Antibody-Drug Conjugate) Development: Serving as a critical linker for site-specific conjugation of cytotoxic payloads to monoclonal antibodies in cancer therapy research.
• Biomaterial and Surface Functionalization: Modifying nanoparticles, hydrogels, or sensor surfaces with bioactive ligands for targeted drug delivery or biosensing.
• Proteomics and Biomarker Discovery: Facilitating the enrichment and identification of azide-tagged biomolecules in complex biological samples.
• In Vivo Imaging Agent Synthesis: Conjugating imaging probes (e.g., PET, fluorescence) to targeting vectors for non-invasive diagnostic imaging.
• Oligonucleotide Modification: Labeling DNA, RNA, or oligonucleotide analogs for genetic analysis, FISH probes, or therapeutic oligonucleotides.
• Polymer Chemistry: Incorporating functional handles into polymers for creating smart, responsive materials with biological activity.
• Chemical Biology Tool Development: Creating activity-based probes and other research tools to study enzyme function and cellular processes.

Basic Information

Product Name	Dbco-Nhs Ester
CAS No.	1353016-71-3
Molecular Formula	C26H24N2O5
Molecular Weight	444.48 g/mol
Synonyms	DBCO-Sulfo-NHS Ester; Dibenzocyclooctyne-sulfo-N-hydroxysuccinimide ester; DBCO-SE; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl N-succinimidyl carbonate; Sulfo-DBCO-NHS Ester; Azadibenzocyclooctyne-NHS ester; DBCO-PEG4-NHS Ester (common related analog); Click Chemistry DBCO Linker
EINECS	Contact for details

Quality Control

Our Dbco-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure it meets the high standards required for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For optimal performance, use anhydrous solvents and store aliquots under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.