Description

Azido-Peg4-Pfp Ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide group and a pentafluorophenyl (PFP) ester. This compound is specifically engineered for efficient, chemoselective bioconjugation, enabling the stable linkage of biomolecules under mild conditions. It is an essential reagent for researchers and manufacturers in biopharmaceutical development, proteomics, and advanced materials science, facilitating the creation of antibody-drug conjugates (ADCs), peptide modifications, and functionalized surfaces.

Application

• Bioconjugation and ADC (Antibody-Drug Conjugate) Development: The PFP ester reacts efficiently with primary amines on antibodies or proteins, while the azide group enables subsequent click chemistry (e.g., CuAAC or SPAAC) for precise drug payload attachment.
• Peptide and Protein Modification: Used to install PEG spacers or functional tags onto peptides and proteins to enhance solubility, stability, and pharmacokinetic properties.
• Surface Functionalization and Material Science: Employed to modify nanoparticles, biosensors, and polymeric materials with bio-recognition elements for diagnostic and therapeutic applications.
• Polymer Chemistry: Serves as a versatile building block for synthesizing functionalized PEG-based polymers and hydrogels with tailored properties.
• Chemical Biology and Probe Synthesis: Facilitates the creation of activity-based probes and imaging agents for studying biological processes.
• Diagnostic Reagent Manufacturing: Used in the production of conjugated antigens and antibodies for immunoassays and lateral flow tests.

Basic Information

Product Name	Azido-Peg4-Pfp Ester
CAS No.	1353012-00-6
Molecular Formula	C23H30F5N3O7
Molecular Weight	555.5 g/mol
Synonyms	Azido-PEG4-PFP ester; 4-Azidobutyric acid-PEG4-PFP ester; Pentafluorophenyl 4-azidobutanoate-PEG4 ester; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-[2-[2-(4-azidobutanoyloxy)ethoxy]ethoxy]ethoxy]ethoxy]propanoate; Azido-PEG4-OSu (PFP ester variant); N3-PEG4-PFP ester; Heterobifunctional PEG crosslinker, Azide-PFP; 1-((14-Azido-3,6,9,12-tetraoxatetradecan-1-oyl)oxy)pyrrolidine-2,5-dione, Pentafluorophenyl ester derivative
EINECS	Contact for details

Quality Control

Our Azido-Peg4-Pfp Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistent performance for critical research and production applications. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided with every shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous liquid or oil
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.