Description

Ald-Peg4-Nhs Ester CAS NO 1353011-74-1 is a heterobifunctional crosslinker featuring an aldehyde group and an N-hydroxysuccinimide (NHS) ester, connected by a tetraethylene glycol (PEG4) spacer. This compound is engineered to facilitate the stable, site-specific conjugation of biomolecules, such as proteins and peptides, under mild aqueous conditions. It is an essential reagent for researchers and manufacturers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic assay formulation, where precise and efficient linking is critical.

Application

• Bioconjugation and Protein Labeling: For the covalent attachment of fluorescent dyes, enzymes, or other probes to antibodies, proteins, or peptides via amine groups.
• Antibody-Drug Conjugate (ADC) Synthesis: Serves as a key linker-payload component, connecting cytotoxic drugs to monoclonal antibodies through stable bonds.
• Surface Immobilization and Bioprobe Development: Used to functionalize sensor chips, microplates, or nanoparticles with biomolecules for biosensing and diagnostic applications.
• Polymer and Biomaterial Modification: Enables the introduction of aldehyde or amine-reactive functionalities into hydrogels and other polymeric materials.
• Crosslinking for Structural Studies: Applied in mass spectrometry and structural biology to study protein-protein interactions and complex architectures.
• Diagnostic Reagent Manufacturing: A critical component in the production of conjugated antigens, antibodies, and other reagents for immunoassays (ELISA, lateral flow tests).

Basic Information

Product Name	Ald-Peg4-Nhs Ester
CAS No.	1353011-74-1
Molecular Formula	C21H33NO10
Molecular Weight	459.49 g/mol
Synonyms	5-(((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid, PEG4 aldehyde NHS ester; Aldehyde-PEG4-NHS ester; 4-Formylbenzyl-PEG4-NHS ester; NHS-PEG4-CHO; (4-Formylbenzyloxy)-PEG4-NHS ester; 2,5-Dioxopyrrolidin-1-yl 4-((4-formylbenzyl)oxy)-4-oxobutanoate (PEG4 spacer); N-(4-Formylbenzyloxy)succinimidyl-PEG4-ester
EINECS	Contact for details

Quality Control

Our Ald-Peg4-Nhs Ester is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and development applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity assessment and NMR for structural confirmation. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.