Description

Ortho-Nitroveratryloxycarbonyl-Q-Rhodamine-5-Succinimidyl Ester is a highly specialized, photolabile protecting group reagent designed for advanced bioconjugation and labeling applications. This compound is critical for researchers requiring precise, light-triggered control over the attachment of fluorescent rhodamine probes to biomolecules like peptides, proteins, and oligonucleotides. It is an essential tool for scientists in pharmaceutical R&D, chemical biology, and advanced diagnostics who are developing next-generation targeted imaging agents and therapeutic conjugates.

Application

• Photocleavable Fluorescent Labeling: Site-specific conjugation of Q-Rhodamine-5 fluorophores to biomolecules for controlled release or activation studies.
• Advanced Bioconjugation Chemistry: Synthesis of caged fluorescent probes for super-resolution microscopy (e.g., PALM, STORM) and single-molecule tracking.
• Prodrug and Theranostic Development: Construction of light-activatable drug delivery systems and diagnostic-therapeutic combinations (theranostics).
• Peptide and Protein Modification: Selective labeling of amino groups (e.g., lysine residues or N-termini) with a fluorescence quencher that can be removed upon irradiation.
• Nucleic Acid Probe Synthesis: Preparation of photocontrollable fluorescent DNA or RNA probes for real-time PCR, in situ hybridization, and gene expression analysis.
• Materials Science and Surface Functionalization: Creating light-responsive fluorescent coatings or sensors on nanoparticles and other solid supports.

Basic Information

Product Name	Ortho-Nitroveratryloxycarbonyl-Q-Rhodamine-5-Succinimidyl Ester
CAS No.	1352809-17-6
Molecular Formula	C41H38N4O11
Molecular Weight	762.77 g/mol
Synonyms	NVOC-Q-Rhodamine-5-SE; o-Nitroveratryloxycarbonyl-Q-Rhodamine-5-NHS Ester; Q-Rhodamine 5-(o-Nitroveratryloxycarbonyl)succinimidyl ester; 2-(2-Nitro-4,5-dimethoxybenzyloxycarbonyl)-Q-Rhodamine-5-succinimidyl ester; Photocleavable Q-Rhodamine 5 NHS ester; Light-sensitive Rhodamine labeling reagent; NVOC-caged Rhodamine succinimidyl ester
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with the high-purity standards required for sensitive research and development applications. Each batch is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and functional integrity. Certificates of Analysis (COA) detailing lot-specific results are available upon request to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. Due to its hygroscopic and strictly light-sensitive nature, this material should be handled under inert atmosphere (argon or nitrogen) when possible and allowed to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Red to purple solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.