Description

Cyanine 3 Bihexanoic Acid Dye, Succinimidyl Ester, Potassium Salt is a reactive, water-soluble near-infrared fluorescent labeling reagent. This compound is engineered for high-efficiency conjugation to primary amines, offering superior stability and brightness for demanding bioimaging and diagnostic applications. It is an essential tool for researchers and manufacturers in the fields of bioconjugation, flow cytometry, in vivo imaging, and microarray development, where reliable and intense fluorescence signals are critical.

Application

• Antibody and Protein Labeling: Conjugation to monoclonal antibodies, streptavidin, and other proteins for fluorescence-activated cell sorting (FACS) and immunofluorescence.
• Nucleic Acid Probes: Labeling of oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• In Vivo Imaging: Tagging of peptides, antibodies, or nanoparticles for non-invasive deep-tissue imaging in preclinical research.
• Microarray and Biosensor Fabrication: Immobilization of biomolecules on solid surfaces for high-throughput screening and diagnostic devices.
• Cell Tracing and Tracking: Long-term labeling of cells for migration and proliferation studies.
• Drug Delivery Research: Conjugation to drug carriers or targeting ligands to visualize biodistribution and pharmacokinetics.

Basic Information

Product Name	Cyanine 3 Bihexanoic Acid Dye, Succinimidyl Ester, Potassium Salt
CAS No.	1311966-47-8
Molecular Formula	C41H49KN2O9
Molecular Weight	752.93 g/mol
Synonyms	Cy3 Bihexanoic Acid, SE, Potassium Salt; Sulfo-Cyanine3 NHS Ester; Cyanine3 Bihexanoic Acid N-Hydroxysuccinimide Ester Potassium Salt; Cy3 Bihexanoic Acid Succinimidyl Ester Potassium Salt; 1-(6-{2-[(1E,3E)-5-{2-[3-(2,5-Dioxopyrrolidin-1-yl)oxy]-3-oxopropyl]-1,3,3-trimethylindolin-2-ylidene}ethylidene)-1-cyclohex-2-en-1-ylidene}ethyl)-3,3-dimethyl-1-(5-sulfonatopentyl)indolium, inner salt, potassium salt; Cy3™-type NHS ester; Water-soluble Cy3 NHS ester
EINECS	Contact for details

Quality Control

Our Cyanine 3 Bihexanoic Acid Dye, Succinimidyl Ester, Potassium Salt is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure consistency and performance. Certificates of Analysis (COA) with detailed specifications are provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark green to purple solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Extinction Coefficient (ε)	≥ 150,000 cm⁻¹M⁻¹ at λmax
Fluorescence Quantum Yield	Contact for details
Water Content (KF)	≤ 5.0%
Single Spot (TLC)	One major spot

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.