Description

Cy3.5 CAS NO 1284240-77-2 is a high-purity, near-infrared fluorescent cyanine dye derivative widely used as a critical labeling reagent in advanced life science research. Its core value lies in providing exceptional photostability and high fluorescence quantum yield, enabling sensitive and reliable detection in complex biological assays. This product is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic sectors who require precise and stable fluorescent tags for applications such as nucleic acid labeling, protein conjugation, and in vivo imaging.

Application

• Nucleic Acid Labeling and Detection: Used for fluorescent labeling of oligonucleotides (DNA, RNA) for applications in fluorescence in situ hybridization (FISH), microarray analysis, and real-time PCR.
• Protein and Antibody Conjugation: Serves as a reactive dye for labeling proteins, antibodies, and peptides for use in fluorescence microscopy, flow cytometry (FACS), and Western blotting.
• In Vivo and In Vitro Imaging: Employed as a contrast agent in small animal imaging and cellular imaging due to its near-infrared emission, which minimizes background autofluorescence.
• Diagnostic Assay Development: A key component in the development of fluorescent immunoassays, lateral flow tests, and other clinical diagnostic platforms.
• Biomolecular Interaction Studies: Utilized in fluorescence resonance energy transfer (FRET) and other biophysical techniques to study molecular interactions and conformational changes.
• Cell Tracing and Proliferation Assays: Applied for long-term cell tracking and monitoring cell proliferation in biomedical research.

Basic Information

Product Name	Cy3.5
CAS No.	1284240-77-2
Molecular Formula	C40H45KN2O9S2
Molecular Weight	809.02 g/mol
Synonyms	Cyanine3.5; Cyanine 3.5 carboxylic acid; Sulfo-Cyanine3.5 acid; Cy3.5 NHS ester (reactive form precursor); 2-((1E,3E)-5-(1-(5-Carboxypentyl)-3,3-dimethyl-5-sulfo-3H-indol-1-ium-2-yl)penta-1,3-dien-1-yl)-1-ethyl-3,3-dimethyl-3H-indol-1-ium-5-sulfonate; Potassium (E)-1-(6-carboxyhexyl)-2-((E)-3-((E)-1-ethyl-3,3-dimethyl-5-sulfoindolin-2-ylidene)prop-1-en-1-yl)-3,3-dimethyl-5-sulfo-3H-indol-1-ium-1-ide
EINECS	Contact for details

Quality Control

Our Cy3.5 is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment, spectrophotometric analysis for molar extinction coefficient verification, and functional testing for labeling efficiency. Certificates of Analysis (COA) detailing all specifications and test results are provided with every shipment. We adhere to relevant industry guidelines to support our customers' research and development under quality frameworks like ISO 9001.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term stability, store under inert atmosphere or desiccant.

Specification

Item	Specification
Appearance	Dark green to blue-green solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	581 ± 3 nm
Emission Maximum (λem)	596 ± 3 nm
Extinction Coefficient (ε)	≥ 150,000 cm⁻¹M⁻¹ at λmax
Moisture Content (KF)	≤ 5.0 %
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.