Description

Cyanine5 Azide CAS NO 1267539-32-1 is a near-infrared fluorescent dye functionalized with a reactive azide group. This compound is essential for bioorthogonal click chemistry, enabling the specific labeling and visualization of biomolecules in complex biological systems without interfering with native biochemical processes. It is a critical reagent for researchers and developers in bioconjugation, bioimaging, flow cytometry, and in vivo diagnostics, providing high sensitivity and low background signal.

Application

• Bioconjugation and Probe Synthesis: Used in copper-free click chemistry reactions to label proteins, antibodies, oligonucleotides, and other biomolecules with a near-infrared fluorophore.
• Super-Resolution and Fluorescence Microscopy: Enables high-resolution imaging of cellular structures and dynamic processes due to its excellent photostability and far-red emission.
• In Vivo Imaging: Ideal for whole-animal imaging applications as its emission wavelength penetrates tissue more effectively, minimizing autofluorescence.
• Flow Cytometry and Cell Sorting: Facilitates the detection and isolation of specific cell populations labeled via click chemistry.
• Diagnostic Assay Development: Serves as a key component in developing sensitive fluorescent immunoassays and molecular diagnostic tests.
• Materials Science: Employed to functionalize nanoparticles, polymers, and surfaces for creating smart materials with optical reporting capabilities.

Basic Information

Product Name	Cyanine5 Azide
CAS No.	1267539-32-1
Molecular Formula	C37H45N6O+
Molecular Weight	613.80 g/mol
Synonyms	Cy5 Azide; Sulfo-Cyanine5 Azide (common variant); Cy5 N3; 1-(5-Carboxypentyl)-2-((1E,3E)-5-(1-(6-azidohexyl)-3,3-dimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-3,3-dimethyl-3H-indol-1-ium; NIR Fluorophore Azide; Azide-functionalized Cyanine5; Near-IR Click Chemistry Dye
EINECS	Contact for details

Quality Control

Every batch of Cyanine5 Azide is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We ensure high batch-to-batch consistency and purity suitable for sensitive life science applications. A Certificate of Analysis (COA) detailing purity, identity, and performance characteristics is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption (λmax)	646 - 652 nm
Emission (λmax)	662 - 668 nm
Extinction Coefficient (ε)	≥ 250,000 L·mol⁻¹·cm⁻¹
Water Content (KF)	≤ 2.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.