Description

1-{12-[4-(Dipyrrometheneboron Difluoride)Butanoyl]Amino}Dodecanoyl-2-Hydroxy-Sn-Glycero-3-Phosphate (Ammonium Salt) is a sophisticated, fluorescently labeled phospholipid derivative designed for advanced research and diagnostic applications. This compound matters because it integrates a bright, photostable BODIPY fluorophore with a biologically relevant phospholipid structure, enabling precise membrane labeling and tracking. It is essential for researchers and developers in biochemistry, cell biology, and pharmaceutical sciences who require high-purity probes for studying lipid dynamics, membrane trafficking, and cellular imaging.

Application

• Fluorescent Cell Membrane Labeling: Used as a vital probe for visualizing and tracking plasma membrane and intracellular membrane dynamics in live-cell imaging.
• Lipid Raft and Microdomain Studies: Employed in research to investigate the organization and function of lipid rafts and membrane microdomains.
• Liposome and Model Membrane Research: Incorporated into liposomes and supported lipid bilayers (SLBs) for biophysical studies of membrane properties and protein-lipid interactions.
• Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Serves as a stable fluorescent marker for cell population analysis and sorting based on membrane characteristics.
• Drug Delivery System Development: Utilized in the design and evaluation of targeted drug delivery carriers to monitor carrier-cell interactions and intracellular fate.
• High-Content Screening (HCS) Assays: Acts as a key reagent in automated imaging assays for drug discovery and toxicology studies involving membrane targets.

Basic Information

Product Name	1-{12-[4-(Dipyrrometheneboron Difluoride)Butanoyl]Amino}Dodecanoyl-2-Hydroxy-Sn-Glycero-3-Phosphate (Ammonium Salt)
CAS No.	1246355-62-3
Molecular Formula	C₃₈H₆₆BF₂N₃O₉P
Molecular Weight	806.71 g/mol
Synonyms	BODIPY® FL C12-HPC; 1-(12-{4-[Difluoro(1-hydroxy-3-oxo-3H-isoindol-2-yl)boranyl]butanoyl}amino)dodecanoyl-2-hydroxy-sn-glycero-3-phosphate ammonium salt; BODIPY FL DHPE; Fluorescent Phosphatidylcholine Analog; BODIPY® 500/510 C12-HPC; 2-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)-1-Hexadecanoyl-sn-Glycero-3-Phosphocholine (common analog name); BODIPY® FL C12-GPI (related glycolipid analog); LysoPB PC (BODIPY FL C12)
EINECS	Contact for details

Quality Control

Our high-purity fluorescent lipids are manufactured under controlled conditions and undergo rigorous analytical testing to ensure batch-to-batch consistency and performance. Each lot is characterized using advanced techniques including HPLC, mass spectrometry, and NMR to confirm identity, purity, and fluorescent properties. A comprehensive Certificate of Analysis (COA) detailing assay, impurity profile, and functional performance data is provided with every shipment to support your critical research and development work.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. Solutions prepared in organic solvents should be aliquoted and stored under inert atmosphere (argon or nitrogen) at -20°C in the dark.

Specification

Item	Specification
Appearance	Orange to red solid or film
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum consistent with molecular ion [M-H]⁻
Purity (HPLC)	≥ 95% (Area % at specified wavelength)
Assay	≥ 90% (by phosphorus analysis)
Fluorescence Properties	Excitation/Emission maxima consistent with BODIPY FL dye (~503/512 nm in lipid membranes)
Solubility	Soluble in chloroform, methanol, DMSO; forms micelles in aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.