Description

Bodipy C12-Ceramide CAS NO 1246355-58-7 is a fluorescently labeled lipid derivative, specifically a ceramide analog tagged with a boron-dipyrromethene (BODIPY) fluorophore on a C12 acyl chain. This compound is a critical tool for real-time visualization and tracking of lipid metabolism, membrane trafficking, and intracellular signaling pathways in live-cell and fixed-cell imaging. It is essential for researchers and developers in cell biology, lipidomics, and pharmaceutical development who require high-purity, photostable probes for advanced fluorescence microscopy and flow cytometry applications.

Application

• Live-Cell Imaging of Lipid Trafficking: Visualizing the dynamics of ceramide and sphingolipid transport within cellular organelles such as the Golgi apparatus and endoplasmic reticulum.
• Membrane Biophysics Studies: Investigating lipid raft formation, membrane fluidity, and phase separation in model and cellular membranes.
• Apoptosis & Cell Signaling Research: Tracking the role of ceramide in programmed cell death and stress response pathways using fluorescence microscopy.
• Flow Cytometry & FACS: Staining and sorting specific cell populations based on lipid content or metabolic activity.
• Drug Discovery Screening: Serving as a fluorescent probe in high-content screening assays to identify compounds that modulate lipid metabolism or ceramide-related pathways.
• Neuroscience Research: Studying the role of sphingolipids in neuronal membrane structure, synaptic function, and neurodegenerative disease models.

Basic Information

Product Name	Bodipy C12-Ceramide
CAS No.	1246355-58-7
Molecular Formula	C44H72BF2N3O4
Molecular Weight	755.87 g/mol
Synonyms	BODIPY® FL C12-Ceramide; BODIPY FL C12-Ceramide; N-[(1E,3R)-1,3-Dihydroxy-4,4-difluoro-1,3,5,7-tetramethyl-8-(2-methyl-1-oxododecyl)-4-bora-3a,4a-diaza-s-indacen-2-yl]dodecanamide; BODIPY 500/510 C12-Ceramide complex; Fluorescent Ceramide BODIPY FL C12; Ceramide BODIPY FL C12; BODIPY-FL-Ceramide
EINECS	Contact for details

Quality Control

Our Bodipy C12-Ceramide is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high performance in sensitive biological assays. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and fluorescence properties. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/emission profiles, and functional performance data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature before opening to minimize condensation and moisture uptake. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red solid or film
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Excitation/Emission Maxima	~500 nm / ~510 nm
Solubility	Soluble in DMSO, DMF, ethanol
Loss on Drying	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.