Description

5-Cholesten-3-Ol 12-[(7-Nitro-2-1,3-Benzoxadiazol-4-Yl)Amino]Dodecanoate is a specialized fluorescent cholesterol derivative, designed for advanced research and diagnostic applications. Its core value lies in its ability to act as a highly sensitive fluorescent probe for tracking cholesterol metabolism, transport, and localization in biological systems. This compound is essential for researchers and developers in pharmaceutical R&D, cell biology, and diagnostic assay development who require precise tools for lipid visualization and analysis.

Application

• Fluorescent Labeling Agent: Used as a vital probe for labeling and visualizing cholesterol and its esters in live-cell imaging and fluorescence microscopy studies.
• Lipid Metabolism Research: A critical tool for investigating cholesterol uptake, intracellular trafficking, and esterification pathways in various cell types.
• Diagnostic Assay Development: Employed in the development of high-sensitivity fluorescence-based assays for clinical diagnostics and biochemical analysis.
• Membrane Biology Studies: Facilitates the study of lipid raft dynamics, membrane fluidity, and the role of cholesterol in membrane structure and function.
• Pharmaceutical Screening: Used in drug discovery platforms to screen for compounds that modulate cholesterol homeostasis and related metabolic disorders.
• Biochemical Reagent: Serves as a key reagent in enzymatic studies related to cholesterol-modifying enzymes like ACAT (Acyl-CoA cholesterol acyltransferase).

Basic Information

Product Name	5-Cholesten-3-Ol 12-[(7-Nitro-2-1,3-Benzoxadiazol-4-Yl)Amino]Dodecanoate
CAS No.	1246303-05-8
Molecular Formula	C46H71N3O5
Molecular Weight	746.08 g/mol
Synonyms	NBD-Cholesteryl Dodecanoate; NBD-Cholesteryl Laurate; 12-[(7-Nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanoic Acid Cholesteryl Ester; NBD-C12-Cholesterol; Fluorescent Cholesterol Laurate; Cholesteryl NBD Dodecanoate; NBD-labeled Cholesterol Ester; 4-(12-((Cholest-5-en-3β-yloxy)carbonyl)dodecylamino)-7-nitrobenz-2-oxa-1,3-diazole
EINECS	Contact for details

Quality Control

Our 5-Cholesten-3-Ol 12-[(7-Nitro-2-1,3-Benzoxadiazol-4-Yl)Amino]Dodecanoate is produced under strict quality management systems to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to confirm identity and purity. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. This product is strictly light-sensitive (protect from all light exposure). For long-term stability, store at -20°C or below under desiccant. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. Store away from strong acids and oxidizing agents.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMSO, DMF, chloroform
Fluorescence Properties	Ex/Em ~465/535 nm (in lipid membrane)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.